| Full text | |
| Author(s): |
Caetano-Silva, Maria Elisa
[1]
;
Simabuco, Fernando Moreira
[2]
;
Neves Bezerra, Rosangela Maria
[2]
;
da Silva, Daniele Cristina
[1]
;
Barbosa, Eder Alves
[3, 4]
;
Moreira, Daniel Carneiro
[4]
;
Brand, Guilherme Dotto
[3]
;
de Souza de Almeida Leite, Jose Roberto
[4]
;
Bertoldo Pacheco, Maria Teresa
[1]
Total Authors: 9
|
| Affiliation: | [1] ITAL, Inst Food Technol, Ctr Food Sci & Qual, CCQA, BR-13070178 Campinas - Brazil
[2] Univ Estadual Campinas, UNICAMP, Multidisciplinary Lab Food & Hlth, LABMAS, Sch Appl Sci, FCA, BR-13484350 Limeira - Brazil
[3] Univ Brasilia, Lab Synth & Anal Biomol, Inst Chem, IQ, BR-70910900 Brasilia, DF - Brazil
[4] Univ Brasilia, Fac Med, Morphol Area, Res Ctr Morphol & Appl Immunol, NuPMIA, UnB, BR-70910900 Brasilia, DF - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Journal of Agricultural and Food Chemistry; v. 68, n. 44, p. 12433-12443, NOV 4 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
This study aims to isolate metal-binding peptides and synthesize promising amino acid sequences to potentially act as neuroprotective compounds in the future, targeting different mechanisms. Fractions of whey metal-binding peptides (Cu, Fe, and Zn) isolated by immobilized metal affinity chromatography showed different amino acid profiles according to the metal. The Cu-binding peptides presented roughly twofold increase in the in vitro antioxidant, as assessed by oxygen radical absorbance capacity and anticholinesterase activities over the hydrolysate. This is probably because of the higher concentration of aromatic and basic residues, the latter being crucial for binding to the anionic sites of acetylcholinesterase. Six peptide sequences were synthesized based on the metal-binding sites, molecular mass, hydrophobicity, and bioactivity probability. Among the synthetic peptides, the VF dipeptide stood out both for its in vitro antioxidant and anticholinesterase activities. This peptide, as well as the fraction of Cu-binding peptides, should be further studied because it may act through different mechanisms related to neurodegenerative diseases, in addition to the chelation of the excess of metals in the central nervous system. (AU) | |
| FAPESP's process: | 18/14818-9 - Study of molecular targets important for the control of cancer metabolism: the mTOR/S6K pathway as a central role |
| Grantee: | Fernando Moreira Simabuco |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 17/16977-4 - Obtaining and characterizing peptides with neuroprotective activity |
| Grantee: | Maria Elisa Caetano Silva Abreu |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/06457-0 - Leaves and stalks of beet (Beta vulgaris L.): characterization and effects of the antioxidant capacity in obese and dyslipidemic |
| Grantee: | Rosângela Maria Neves Bezerra |
| Support Opportunities: | Regular Research Grants |