Regulation of Anti-Apoptotic SOD2 and BIRC3 in Periodontal Cells and Tissues

Autor(es):Mostrar menos -

Rath-Deschner, Birgit ^[1] ; Nogueira, Andressa Vilas Boas ^[2] ; Memmert, Svenja ^{[3, 1]} ; Nokhbehsaim, Marjan ^[3] ; Augusto Cirelli, Joni ^[4] ; Eick, Sigrun ^[5] ; Miosge, Nicolai ^[6] ; Kirschneck, Christian ^[7] ; Kesting, Marco ^[8] ; Deschner, James ^[2] ; Jaeger, Andreas ^[1] ; Damanaki, Anna ^[2]

Afiliação do(s) autor(es)

^[1] Univ Bonn, Ctr Dentomaxillofacial Med, Dept Orthodont, D-53111 Bonn - Germany

^[2] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Dept Periodontol & Operat Dent, D-55131 Mainz - Germany

^[3] Univ Bonn, Ctr Dentomaxillofacial Med, Sect Expt Dentomaxillofacial Med, D-53111 Bonn - Germany

^[4] Sao Paulo State Univ, UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP - Brazil

^[5] Zahnmedizin Kliniken, Lab Oral Microbiol, Dept Periodontol, CH-53010 Bern - Switzerland

^[6] Univ Med Ctr, Dept Prosthodont, D-37075 Gottingen - Germany

^[7] Univ Regensburg, Dept Orthodont, D-93053 Regensburg - Germany

^[8] Friedrich Alexander Univ Erlangen Nurnberg FAU, Dept Oral & Maxillofacial Surg, D-91054 Erlangen - Germany

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 2 JAN 2021.
• Citações Web of Science: 0

Resumo

The aim of the study was to clarify whether orthodontic forces and periodontitis interact with respect to the anti-apoptotic molecules superoxide dismutase 2 (SOD2) and baculoviral IAP repeat-containing protein 3 (BIRC3). SOD2, BIRC3, and the apoptotic markers caspases 3 (CASP3) and 9 (CASP9) were analyzed in gingiva from periodontally healthy and periodontitis subjects by real-time PCR and immunohistochemistry. SOD2 and BIRC3 were also studied in gingiva from rats with experimental periodontitis and/or orthodontic tooth movement. Additionally, SOD2 and BIRC3 levels were examined in human periodontal fibroblasts incubated with Fusobacterium nucleatum and/or subjected to mechanical forces. Gingiva from periodontitis patients showed significantly higher SOD2, BIRC3, CASP3, and CASP9 levels than periodontally healthy gingiva. SOD2 and BIRC3 expressions were also significantly increased in the gingiva from rats with experimental periodontitis, but the upregulation of both molecules was significantly diminished in the concomitant presence of orthodontic tooth movement. In vitro, SOD2 and BIRC3 levels were significantly increased by F. nucleatum, but this stimulatory effect was also significantly inhibited by mechanical forces. Our study suggests that SOD2 and BIRC3 are produced in periodontal infection as a protective mechanism against exaggerated apoptosis. In the concomitant presence of orthodontic forces, this protective anti-apoptotic mechanism may get lost. (AU)

• Processo FAPESP: 17/07137-2 - Efeitos da obesidade nos tecidos periodontais sujeitos a forças biomecânicas
• Beneficiário: Joni Augusto Cirelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular