Interference with the renin-angiotensin system reduces the palatability of 0.3 M NaCl in sodium-deplete rats

Autor(es)

Zenatti, A. A. ^[1] ; Pereira Jr, E. D. ; Possari, J. ^[2] ; Andrade, C. A. F. ^[2] ; Menani, V, J. ; De Luca Jr, L. A.

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Dent FOAr, Dept Physiol & Pathol, UNESP, Araraquara, SP - Brazil

^[2] Pereira Jr, Jr., E. D., Sao Paulo State Univ, Sch Dent FOAr, Dept Physiol & Pathol, UNESP, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APPETITE; v. 158, MAR 1 2021.
• Citações Web of Science: 0

Resumo

The renin-angiotensin system (RAS) controls hypertonic NaCl intake driven by sodium appetite. Here we investigated whether the antagonism of RAS interferes with hedonic and aversive omfacial motor responses, or palatability, to intraoral infusion of 0.3 M NaCl (hNaCl). Adult rats were depleted of sodium by combined sc injection of furosemide and 24 h removal of ambient sodium. In experiment 1, losartan (AT1 angiotensin II receptor antagonist, intracerebroventricular, 200 mu g/mu l), produced a three-fold increase in aversive orofacial motor responses to hNaCl. Losartan also suppressed hNaCl intake recorded immediately thereafter. In experiment 2, each animal had repeated recordings of hNaCl intake and omfacial responses to hNaCl distributed for 180 min. Paired recordings of intake and orofacial responses occurred within five successive blocks after the recordings of only orofacial responses when the animals were still sodium deplete (block zero). Captopril (angiotensin converting enzyme blocker, intraperitoneal, 30 mg/kg) inhibited by 75% the hedonic orofacial responses to hNaCl in blocks zero and 1. The hedonic responses to captopril remained the same throughout blocks, but became similar to vehicle from blocks 2 to 5. There was no difference in aversive responses to 0.3 M NaCl between captopril and vehicle. Captopril produced a 70-100% inhibition of hNaCl intake in blocks 1 to 5. The results suggest that angiotensin II acts in the brain increasing the palatability of hypertonic sodium during the consummatory phase of sodium appetite. (AU)

• Processo FAPESP: 15/23467-7 - Fisiopatologia experimental: mecanismos centrais de controle cardiovascular e respiratório envolvidos em modelos experimentais de hipertensão e obesidade
• Beneficiário: Eduardo Colombari
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/50770-1 - Mecanismos neurais de regulação do equilíbrio hidroeletrolítico e controle cardiorrespiratório
• Beneficiário: José Vanderlei Menani
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/01220-0 - Papel da ativação dos adrenoceptores alfa2 nas respostas orofaciais ao sabor salgado e a capsaicina em ratos depletados de sódio
• Beneficiário: Juliana Possari
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/20500-3 - Importância do núcleo parabraquial lateral e receptores angiotensinérgicos prosencefálicos no controle do equilíbrio hidromineral durante hipertensão essencial.
• Beneficiário: Carina Aparecida Fabrício de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular