Smoking load reduction is insufficient to downregulate miR-301b, a lung cancer promoter

Autor(es)

Arcas, Camila dos Santos ^[1] ; Lin-Wang, Hui Tzu ^[2] ; Kikuchi Umeda, Iracema Ioco ^{[2, 1]} ; de Sousa, Marcio Goncalves ^[2] ; Odagiri Utiyama, Daniela Mitiyo ^[3] ; Mansur, Antonio de Padua ^[4] ; Macchione, Mariangela ^[5] ; Hirata, Mario Hiroyuki ^[6] ; Nakagawa, Naomi Kondo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Physiotherapy, LIM-54, Av Dr Arnaldo, 455 Room 1150, BR-01246930 Sao Paulo, SP - Brazil

^[2] Dante Pazzanese Inst Cardiol Sao Paulo State, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Med Rehabil Lucy Montoro Inst, Fac Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Cardiopneumol, Fac Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Dept Pathol, Fac Med, LIM05, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 DEC 3 2020.
• Citações Web of Science: 1

Resumo

Several circulating miRNAs identified in the plasma of smokers have been implicated as promoters of nasopharyngeal and lung carcinoma. To investigate the plasma profile of miRNAs in subjects who reduces the number of smoked cigarettes and who quit after six months. We accompanied 28 individuals enrolled in a Smoking Cessation Program over 6 months. At Baseline, clinical characteristics, co-morbidities, and smoking history were similar among subjects. After 6 months, two groups were defined: who successfully quitted smoking (named ``quitters{''}, n=18, mean age 57 years, 11 male) and who reduced the number of cigarettes smoked (20-90%) but failed to quit smoking (named ``smokers{''}, n=10, mean age 52 years, 3 male). No significant clinical changes were observed between groups at baseline and after a 6-month period, however, quitters showed significant downregulations in seven miRNAs at baseline: miR-17 (-2.90 -fold, p=0.029), miR-20a (-3.80-fold, p=0.021); miR-20b (-4.71-fold, p=0.027); miR-30a (-3.95-fold, p=0.024); miR-93 (-3.63-fold, p=0.022); miR-125a(-1.70 -fold, p= 0.038); and miR-195 (-5.37-fold, p=0.002), and after a 6-month period in 6 miRNAs: miR-17 (-5.30-fold, p=0.012), miR-20a (-2.04-fold, p=0.017), miR-20b (-5.44-fold, p=0.017), miR-93 (-4.00-fold, p=0.041), miR-101 (-4.82-fold, p=0.047) and miR-125b (-3.65-fold, p=0.025). Using time comparisons, only quitters had significant downregulation in miR-301b (-2.29-fold, p=0.038) after 6-month. Reductions in the number of smoked cigarettes was insufficient to change the plasma profile of miRNA after 6 months. Only quitting smoking (100% reduction) significantly downregulated miR-301b related to hypoxic conditions, promotion of cell proliferation, decreases in apoptosis, cancer development, and progression as increases in radiotherapy and chemotherapy resistance. (AU)

• Processo FAPESP: 17/21264-7 - Fatores preditores de fraqueza muscular inspiratória em pacientes com insuficiência cardíaca: parâmetros clínicos, funcionais e biomarcadores inflamatórios e de doenças cardiovasculares
• Beneficiário: Naomi Kondo Nakagawa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/13598-1 - Fisioterapia: funcionalidade e estudo celular e biomolecular no tratamento de pacientes com disfunções cardiorrespiratórias
• Beneficiário: Naomi Kondo Nakagawa
• Modalidade de apoio: Auxílio à Pesquisa - Regular