Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules

Autor(es)

Pereira, Melissa Carolina ^[1] ; Nodari, Elen Fernanda ^[1] ; de Abreu, Marina Rodrigues ^[1] ; Paiatto, Lisiery Negrini ^[2] ; Simioni, Patricia Ucelli ^[2] ; Camargo-Mathias, Maria Izabel ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Biol, Inst Biosci, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Genet Evolut & Bioagents, Inst Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental and Applied Acarology; v. 83, n. 3 FEB 2021.
• Citações Web of Science: 0

Resumo

Rhipicephalus sanguineus sensu lato (s.l.), popularly known as `brown dog tick', is the primary vector of pathogens affecting dogs worldwide. To enter the host's organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick's saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 mu g/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks. (AU)

• Processo FAPESP: 19/02831-3 - Resposta dos carrapatos Rhipicephalus sanguineus lato sensu (Acari: Ixodidae) e dos camundongos Mus musculus a exposição aos óleos vegetais de Egletes viscosa (Asteraceae) e Lippia schaueriana (Verbenaceae), potenciais acaricidas naturais
• Beneficiário: Melissa Carolina Pereira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/02999-9 - Ação in vitro e in vivo do ozônio sobre células de linhagem embrionária (RML RSE e RML-15) e fêmeas adultas de carrapatos R. sanguineus s. l. semi ingurgitadas
• Beneficiário: Marina Rodrigues de Abreu
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/20745-6 - Exposição de células da linhagem tumoral J774 (macrófago) ao extrato das glândulas salivares de fêmeas de carrapato Rhipicephalus sanguineus L.S. (Acari: Ixodidae): avaliação morfofisiológica
• Beneficiário: Melissa Carolina Pereira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/02843-8 - Hematofagia em fêmeas de Rhipicephalus sanguineus L. S. (Acari: Ixodidae) e suas relações: bioquímicas (bioativos presentes em extratos) e morfofisiológicas (glândulas salivares e intestino)
• Beneficiário: Elen Fernanda Nodari
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto