Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia

Autor(es)

da Silva, Camila Batista ^{[1, 2]} ; Volpato, Maria Cristina ^[1] ; Muniz, Bruno Vilela ^{[1, 3]} ; dos Santos, Cleiton Pita ^[1] ; Serpe, Luciano ^{[4, 1]} ; Nunes Ferreira, Luiz Eduardo ^{[1, 5]} ; Silva de Melo, Nathalie Ferreira ^{[6, 7]} ; Fraceto, Leonardo Fernandes ^[6] ; Groppo, Francisco Carlos ^[1] ; Franz-Montan, Michelle ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Biosci, Piracicaba, SP - Brazil

^[2] Univ Mogi das Cruzes UMC, Hlth Sci, Mogi Das Cruzes, SP - Brazil

^[3] Itapeva Fac Social & Agr Sci FAIT, Itapeva, SP - Brazil

^[4] Univ Estadual Ponta Grossa, Dept Dent, Ponta Grossa, Parana - Brazil

^[5] Guarulhos Univ UNG, Lab Inflammat & Immunol, Guarulhos, SP - Brazil

^[6] Sao Paulo State Univ, Dept Environm Engn, Sorocaba, SP - Brazil

^[7] Sao Leopoldo Mandic Res Inst, Dept Immunol & Mol Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 2 FEB 11 2021.
• Citações Web of Science: 0

Resumo

To determine whether the permeation capacity and analgesic efficacy of articaine (ATC) could be increased and cytotoxicity decreased by encapsulation in poly(e-caprolactone) nanocapsules (ATC(nano)), aiming at local or topical anesthesia in dentistry. Cellular viability was evaluated (using the MTT test and fluorescence microscopy) after 1 h and 24 h exposure of HaCaT cells to ATC, ATC(nano), ATC with epinephrine (ATC(epi)), and ATC in nanocapsules with epinephrine (ATC(nanoepi)). The profiles of permeation of 2% ATC and 2% ATC(nano) across swine esophageal epithelium were determined using Franz-type vertical diffusion cells. Analgesic efficacy was evaluated with a von Frey anesthesiometer in a postoperative pain model in rats, comparing the 2% ATC, 2% ATC(nano), 2% ATC(epi), and 2% ATC(nanoepi) formulations to 4% ATC(epi) (a commercially available formulation). We show that use of the nanocapsules decreased the toxicity of articaine (P<0.0001) and increased its flux (P = 0.0007). The 2% ATC(epi) and 4% ATC(epi) formulations provided higher analgesia success and duration (P<0.05), compared to 2% ATC, 2% ATC(nano), and 2% ATC(nanoepi). Articaine-loaded poly(e-caprolactone) nanocapsules constitute a promising formulation for intraoral topical anesthesia (prior to local anesthetic injection), although it is not effective when injected in inflamed tissues for pain control, such as irreversible pulpitis. (AU)

• Processo FAPESP: 12/06974-4 - Estudos de desenvolvimento, biocompatibilidade e permeação de formulações em gel de anestésicos locais associados a nanocápsulas de poli-epsilon-caprolactona
• Beneficiário: Michelle Franz Montan Braga Leite
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/02539-1 - Estudos de permeação e biocompatibilidade de formulações de articaína associada a nanocápsulas de poli(epslon-caprolactona).
• Beneficiário: Camila Batista da Silva de Araujo Candido
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/07310-2 - Efeitos modulatórios de anestésicos locais associados a carreadores sobre a produção de mediadores inflamatórios e viabilidade celular em células epiteliais orais e fibroblastos gengivais humanos
• Beneficiário: Francisco Carlos Groppo
• Modalidade de apoio: Auxílio à Pesquisa - Regular