In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection

Autor(es)

Sandoval, Carmen ^[1] ; Araujo, Gabriela ^[1] ; Sosa, Wilfredo ^{[2, 1]} ; Avalos, Sara ^[3] ; Silveira, Fernando ^{[4, 5]} ; Corbett, Carlos ^[1] ; Zuniga, Concepcion ^[6] ; Laurenti, Marcia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Med Sch FMUSP, Dept Pathol, Lab Infect Dis Pathol, Sao Paulo, SP - Brazil

^[2] Natl Autonomous Univ Honduras, Microbiol Res Inst, Tegucigalpa - Honduras

^[3] Natl Autonomous Univ Honduras, Sch Microbiol, Master Program Infect & Zoonot Dis, Tegucigalpa - Honduras

^[4] Fed Univ Para, Inst Trop Med, Belem, Para - Brazil

^[5] Minist Hlth, Dept Parasitol, Evandro Chagas Inst, Secretariat Hlth Surveillance, Belem, Para - Brazil

^[6] School Hosp, Dept Hlth Surveillance, Tegucigalpa - Honduras

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 27, FEB 26 2021.
• Citações Web of Science: 1

Resumo

Abstract Background Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-γ, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-γ. Conclusion Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL. (AU)

• Processo FAPESP: 18/04698-6 - Leishmaniose cutânea não ulcerada ou atípica causada pela Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras: caracterização imuno-histopatológica das lesões de pele com ênfase na participação das células apresentadoras de antígenos
• Beneficiário: Carmen Maria Sandoval Pacheco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50315-0 - Leishmanioses na América Latina: uma perspectiva avançada sobre fatores imunopatogenéticos da infecção cutânea e visceral, imunomoduladores da saliva de vetores flebotomíneos e exo-antígenos imunogênicos de Leishmania (L.) infantum chagasi candidatos à vacina
• Beneficiário: Marcia Dalastra Laurenti
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/24834-9 - Avaliação da evolução da infecção experimental em hamster por parasitos isolados de lesão cutânea atípica e visceral humana causada por Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras
• Beneficiário: Gabriela Venicia Araujo Flores
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/01154-7 - Leishmaniose cutânea não ulcerada ou atípica causada pela Leishmania infantum chagasi no município de Amapala, Valle, Honduras: caracterização imuno-histopatológica das lesões de pele
• Beneficiário: Carmen Maria Sandoval Pacheco
• Modalidade de apoio: Bolsas no Brasil - Mestrado