Enhancement of the Mechanical and Drug-Releasing Properties of Poloxamer 407 Hydrogels with Casein

Autor(es)

Tundisi, Louise Lacalendola ^{[1, 2]} ; Yang, Rong ^[2] ; Borelli, Luiz Phellipe Pozzuto ^[2] ; Alves, Thais ^[3] ; Mehta, Manisha ^[2] ; Chaud, Marco Vinicius ^[3] ; Mazzola, Priscila Gava ^[4] ; Kohane, Daniel S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Pharmaceut Sci, Grad Program Pharmaceut Sci, Campinas, SP - Brazil

^[2] Harvard Med Sch, Lab Biomat & Drug Delivery, Dept Anesthesiol, Div Crit Care Med, Boston Childrens Hosp, Harvard I, Room 914, Boston, MA 02115 - USA

^[3] Univ Sorocaba, LaBNUS Biomat & Nanotechnol Lab, Sorocaba, SP - Brazil

^[4] Univ Campinas Unicamp, Fac Pharmaceut Sci, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICAL RESEARCH; v. 38, n. 3, p. 515-522, MAR 2021.
• Citações Web of Science: 0

Resumo

Purpose Topical therapy of local disease (e.g. skin) is advantageous over oral therapy since there is less systemic drug distribution (so fewer side-effects), no first-pass effect, etc. However, patient compliance with topical therapy can be poor as it may require many applications a day and can last months. Here we propose a topical controlled release formulation with thermoresponsive gelation at body temperature and improved adhesiveness, making it easier to remain in contact with the body. Methods The formulation contains two excipients, poloxamer 407 (P407) and casein. Casein can modify the properties of the hydrogel through molecular entanglement. In addition, tissue reaction and drug release profile were evaluated. Results Changes in casein concentration affected adhesive strength, viscosity, mechanical properties and drug release, presumably by hydrophobic interactions between casein and P407. Two different concentrations of P407 were tested with two different concentrations of casein. Formulations containing 5% and 10% casein released 80% of model drug in 48 h, while formulations without casein released the same fraction in around 24 h hours. Formulations with 10% casein had almost twice the adhesive strength of those without casein. Conclusions Addition of casein modified the mechanical properties and drug release rate of the hydrogel. There was no inflammation or injury after brief exposure in vivo. (AU)

• Processo FAPESP: 17/10728-2 - Encapsulação e modulação de superfície com policaprolactona (PCL): uma nova abordagem para o tratamento de infecções fúngicas tópicas
• Beneficiário: Louise Lacalendola Tundisi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/05421-8 - "Encapsulação e modulação de superfície com policaprolactona (PCL): buscando uma modificação na entrega cutânea de fármaco"
• Beneficiário: Louise Lacalendola Tundisi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 18/07707-6 - Desenvolvimento de forma farmacêutica para tratamento de queimaduras: analgesia, combate à infecções e cicatrização
• Beneficiário: Priscila Gava Mazzola
• Modalidade de apoio: Auxílio à Pesquisa - Regular