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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A comparative analysis of heart microRNAs in vertebrates brings novel insights into the evolution of genetic regulatory networks

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Autor(es):
Nachtigall, Pedro G. [1, 2] ; Bovolenta, Luiz A. [3] ; Patton, James G. [4] ; Fromm, Bastian [5] ; Lemke, Ney [3] ; Pinhal, Danillo [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Especial Toxinol Aplicada LETA, CeTICS, Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Chem & Biol Sci, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Biophys & Pharmacol, Botucatu, SP - Brazil
[4] Vanderbilt Univ, Dept Biol Sci, 221 Kirkland Hall, Nashville, TN 37235 - USA
[5] Stockholm Univ, Wenner Gren Inst MBW, Dept Mol Biosci, Stockholm - Sweden
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: BMC Genomics; v. 22, n. 1 MAR 4 2021.
Citações Web of Science: 0
Resumo

Background During vertebrate evolution, the heart has undergone remarkable changes that lead to morphophysiological differences in the fully formed heart of these species, such as chamber septation, heart rate frequency, blood pressure, and cardiac output volume. Despite these differences, the heart developmental process is guided by a core gene set conserved across vertebrates. Nonetheless, the regulatory mechanisms controlling the expression of genes involved in heart development and maintenance are largely uncharted. MicroRNAs (miRNAs) have been described as important regulatory elements in several biological processes, including heart biology. These small RNA molecules are broadly conserved in sequence and genomic context in metazoans. Mutations may occur in miRNAs and/or genes that contribute to the establishment of distinct repertoires of miRNA-target interactions, thereby favoring the differential control of gene expression and, consequently, the origin of novel phenotypes. In fact, several studies showed that miRNAs are integrated into genetic regulatory networks (GRNs) governing specific developmental programs and diseases. However, studies integrating miRNAs in vertebrate heart GRNs under an evolutionary perspective are still scarce. Results We comprehensively examined and compared the heart miRNome of 20 species representatives of the five major vertebrate groups. We found 54 miRNA families with conserved expression and a variable number of miRNA families with group-specific expression in fishes, amphibians, reptiles, birds, and mammals. We also detected that conserved miRNAs present higher expression levels and a higher number of targets, whereas the group-specific miRNAs present lower expression levels and few targets. Conclusions Both the conserved and group-specific miRNAs can be considered modulators orchestrating the core and peripheral genes of heart GRNs of vertebrates, which can be related to the morphophysiological differences and similarities existing in the heart of distinct vertebrate groups. We propose a hypothesis to explain evolutionary differences in the putative functional roles of miRNAs in the heart GRNs analyzed. Furthermore, we present new insights into the molecular mechanisms that could be helping modulate the diversity of morphophysiology in the heart organ of vertebrate species. (AU)

Processo FAPESP: 13/06864-7 - Análise funcional de microRNAs no desenvolvimento cardíaco de peixes por RNA-seq e genética reversa
Beneficiário:Pedro Gabriel Nachtigall
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/15589-7 - Investigação da composição, funcionalidade e dinâmica evolutiva de microRNAs no genoma de peixes utilizando RNA-Seq e genética reversa
Beneficiário:Danillo Pinhal
Linha de fomento: Auxílio à Pesquisa - Regular