Epitope Mapping of Exposed Tegument and Alimentary Tract Proteins Identifies Putative Antigenic Targets of the Attenuated Schistosome Vaccine

Autor(es):Mostrar menos -

Farias, Leonardo P. ^[1] ; Vance, Gillian M. ^[2] ; Coulson, Patricia S. ^[2] ; Vitoriano-Souza, Juliana ^[1] ; Neto, Almiro Pires da Silva ^[3] ; Wangwiwatsin, Arporn ^[4] ; Neves, Leandro Xavier ^[5] ; Castro-Borges, William ^[5] ; McNicholas, Stuart ^[6] ; Wilson, Keith S. ^[6] ; Leite, Luciana C. C. ^[1] ; Wilson, R. Alan ^[2]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Desenvolvimento Vacinas, Sao Paulo - Brazil

^[2] Univ York, York Biomed Res Inst, York, N Yorkshire - England

^[3] Fundacao Oswaldo Cruz, Inst Goncalo Moniz, Lab Inflamacao & Biomarcadores, Salvador, BA - Brazil

^[4] Wellcome Trust Sanger Inst, Parasite Genom, Cambridge - England

^[5] Univ Fed Ouro Preto, Inst Cincias Exatas & Biol, Ouro Preto - Brazil

^[6] Univ York, York Struct Biol Lab, York, N Yorkshire - England

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 11, MAR 3 2021.
• Citações Web of Science: 5

Resumo

The radiation-attenuated cercarial vaccine remains the gold standard for the induction of protective immunity against Schistosoma mansoni. Furthermore, the protection can be passively transferred to naive recipient mice from multiply vaccinated donors, especially IFNgR KO mice. We have used such sera versus day 28 infection serum, to screen peptide arrays and identify likely epitopes that mediate the protection. The arrays encompassed 55 secreted or exposed proteins from the alimentary tract and tegument, the principal interfaces with the host bloodstream. The proteins were printed onto glass slides as overlapping 15mer peptides, reacted with primary and secondary antibodies, and reactive regions detected using an Agilent array scanner. Pep Slide Analyzer software provided a numerical value above background for each peptide from which an aggregate score could be derived for a putative epitope. The reactive regions of 26 proteins were mapped onto crystal structures using the CCP4 molecular graphics, to aid selection of peptides with the greatest accessibility and reactivity, prioritizing vaccine over infection serum. A further eight MEG proteins were mapped to regions conserved between family members. The result is a list of priority peptides from 44 proteins for further investigation in multiepitope vaccine constructs and as targets of monoclonal antibodies. (AU)

• Processo FAPESP: 12/23124-4 - Investigação dos mecanismos efetores da vacina atenuada de Schistosoma mansoni utilizando Systems Vaccinology
• Beneficiário: Luciana Cezar de Cerqueira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/24832-6 - Desenvolvimento de vacinas baseadas em BCG recombinante: Tuberculose, Pertussis, Pneumococo e Schistosoma
• Beneficiário: Luciana Cezar de Cerqueira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Temático