| Texto completo | |
| Autor(es): |
dos Santos Sampaio, Maria de Fatima
[1, 2]
;
Bastos Boechat, Marcela Santana
[3]
;
Gusman Cunha, Igor Augusto
[2]
;
Pereira, Messias Gonzaga
[3]
;
Coimbra, Norberto Cysne
[1]
;
Giraldi-Guimaraes, Arthur
[2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo FMRP USP, Dept Pharmacol, Lab Neuroanat & Neuropsychobiol, Ribeirao Preto Med Sch, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Northern Fluminense State Univ UENF, Lab Tissue & Cellular Biol, Ctr Biosci & Biotechnol Darcy Ribeiro, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ - Brazil
[3] Northern Fluminense State Univ UENF, Lab Plant Breeding Darcy Ribeiro, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brain Research; v. 1758, MAY 1 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Bone marrow mononuclear cells (BMMCs) have been identified as a relevant therapeutic strategy for the treatment of several chronic diseases of the central nervous system. The aim of this work was to evaluate whether intravenous treatment with BMMCs facilitates the reconnection of lesioned cortico-cortical and cortico-striatal pathways, together with motor recovery, in injured adult Wistar rats using an experimental model of unilateral focal neocortical ischaemia. Animals with cerebral cortex ischaemia underwent neural tract tracing for axonal fibre analysis, differential expression analysis of genes involved in apoptosis and neuroplasticity by RT-qPCR, and motor performance assessment by the cylinder test. Quantitative and qualitative analyses of axonal fibres labelled by an anterograde neural tract tracer were performed. Ischaemic animals treated with BMMCs showed a significant increase in axonal sprouting in the ipsilateral neocortex and in the striatum contralateral to the injured cortical areas compared to untreated rodents. In BMMC-treated animals, there was a trend towards upregulation of the Neurotrophin-3 gene compared to the other genes, as well as modulation of apoptosis by BMMCs. On the 56th day after ischaemia, BMMC-treated animals showed significant improvement in motor performance compared to untreated rats. These results suggest that in the acute phase of ischaemia, Neurotrophin-3 is upregulated in response to the lesion itself. In the long run, therapy with BMMCs causes axonal sprouting, reconnection of damaged neuronal circuitry and a significant increase in motor performance. (AU) | |
| Processo FAPESP: | 17/11855-8 - Estudo da influência de endocanabinoides do corpo estriado e do mesencéfalo ventral na atividade das projeções GABAérgicas neoestriado-nigro-tectais durante as reações de pânico de roedores ameaçados por serpentes peçonhentas |
| Beneficiário: | Norberto Cysne Coimbra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |