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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Neurotrophin-3 upregulation associated with intravenous transplantation of bone marrow mononuclear cells induces axonal sprouting and motor functional recovery in the long term after neocortical ischaemia

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Author(s):
dos Santos Sampaio, Maria de Fatima [1, 2] ; Bastos Boechat, Marcela Santana [3] ; Gusman Cunha, Igor Augusto [2] ; Pereira, Messias Gonzaga [3] ; Coimbra, Norberto Cysne [1] ; Giraldi-Guimaraes, Arthur [2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo FMRP USP, Dept Pharmacol, Lab Neuroanat & Neuropsychobiol, Ribeirao Preto Med Sch, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Northern Fluminense State Univ UENF, Lab Tissue & Cellular Biol, Ctr Biosci & Biotechnol Darcy Ribeiro, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ - Brazil
[3] Northern Fluminense State Univ UENF, Lab Plant Breeding Darcy Ribeiro, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brain Research; v. 1758, MAY 1 2021.
Web of Science Citations: 0
Abstract

Bone marrow mononuclear cells (BMMCs) have been identified as a relevant therapeutic strategy for the treatment of several chronic diseases of the central nervous system. The aim of this work was to evaluate whether intravenous treatment with BMMCs facilitates the reconnection of lesioned cortico-cortical and cortico-striatal pathways, together with motor recovery, in injured adult Wistar rats using an experimental model of unilateral focal neocortical ischaemia. Animals with cerebral cortex ischaemia underwent neural tract tracing for axonal fibre analysis, differential expression analysis of genes involved in apoptosis and neuroplasticity by RT-qPCR, and motor performance assessment by the cylinder test. Quantitative and qualitative analyses of axonal fibres labelled by an anterograde neural tract tracer were performed. Ischaemic animals treated with BMMCs showed a significant increase in axonal sprouting in the ipsilateral neocortex and in the striatum contralateral to the injured cortical areas compared to untreated rodents. In BMMC-treated animals, there was a trend towards upregulation of the Neurotrophin-3 gene compared to the other genes, as well as modulation of apoptosis by BMMCs. On the 56th day after ischaemia, BMMC-treated animals showed significant improvement in motor performance compared to untreated rats. These results suggest that in the acute phase of ischaemia, Neurotrophin-3 is upregulated in response to the lesion itself. In the long run, therapy with BMMCs causes axonal sprouting, reconnection of damaged neuronal circuitry and a significant increase in motor performance. (AU)

FAPESP's process: 17/11855-8 - Study of corpus striatum and ventral mesencephalon endocanabinoid influence on neostriato-nigro-tectal GABAergic pathways during panic reactions of rodents threatened by venomous snakes
Grantee:Norberto Cysne Coimbra
Support Opportunities: Regular Research Grants