Study of corpus striatum and ventral mesencephalon endocanabinoid influence on neostriato-nigro-tectal GABAergic pathways during panic reactions of rodents threatened by venomous snakes

• Grant number: 17/11855-8
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2017 - October 31, 2019
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Norberto Cysne Coimbra
• Grantee: Norberto Cysne Coimbra
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The study of the neuropsychopharmacological bases of the fear- and panic-related behaviour has been the focus of several scientific researches. The mesencephalic tectum (MT) has been considered as responsible for the control of defensive responses evoked during threatening situations. The deep layers of the superior colliculus (dlSC) and the dorsal columns of the periaqueductal grey matter (dPAG) have been considered important structures for the elaboration of innate fear and defensive behaviour. The substantia nigra, pars reticulata (SNpr), is known to receive GABAergic inputs from cautatum-putamen (CPu) and send outputs to the mesencephalic tectum that can work as a sensory channel to dorsal midbrain neurons responsible for the elaboration of defensive responses in more ventral regions of this neural substrate. The dorsal mesencephalon, however, is connected to both compacta and reticulata divisions of the substantia nigra, through inputs rich in endogenous opioid peptides. In addition, both CPu and SNpr are rich in CB1 cannabinoid receptors, with putative involvement in this neural system that is known for these interactive properties with the opioid system, and has been the focus of interest of scientific community. The purpose of the present work is to investigate the role of CB1-cannabinoid receptors of CPu and of opioid and cannabinoide neurotransmnission in SNpr, structures rich in GABAergic neurons, in the modulation of defensive behaviour related to instinctive fear and panic reactions. Thus, it will be studied the effect of microinjections of CB1-cannabinoid receptors selective agonists and antagonists into the CPu or opioid and cannabinoid drugs in SNpr on the defensive behaviour elaborated by dorsal mesencephalon neurons, disinhibited by a GABAA receptor selective blockade in the dlSC/dPAG of Wistar rats confronted with Bothrops alternatus in a polygonal arena for snakes with an artificial burrow. (AU)

Scientific publications

(25)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

B.B. DE PAULA; E.B. VIEIRA-RASTELI; F. CALVO; N.C. COIMBRA; C.R.A. LEITE-PANISSI. Functional activation of the periaqueductal gray matter during conditioned and unconditioned fear in guinea pigs confronted with the Boa constrictor constrictor snake. Brazilian Journal of Medical and Biological Research, v. 55, 2022-02-16. (17/11855-8, 05/00096-1, 12/03798-0, 07/06549-3)

PASCHOALIN-MAURIN, TATIANA; DOS ANJOS-GARCIA, TAYLLON; FALCONI-SOBRINHO, LUIZ LUCIANO; DE FREITAS, RENATO LEONARDO; CYSNE COIMBRA, JADE PISSAMIGLIO; LAURE, CARLOS JULIO; COIMBRA, NORBERTO CYSNE. The Rodent-versus-wild Snake Paradigm as a Model for Studying Anxiety- and Panic-like Behaviors: Face, Construct and Predictive Validities. Neuroscience, v. 369, p. 336-349, JAN 15 2018. (17/11855-8, 07/01174-1, 12/03798-0, 09/17258-5)

FALCONI-SOBRINHO, LUIZ LUCIANO; DOS ANJOS-GARCIA, TAYLLON; DE OLIVEIRA, RICARDO; COIMBRA, NORBERTO CYSNE. Decrease in NMDA receptor-signalling activity in the anterior cingulate cortex diminishes defensive behaviour and unconditioned fear-induced antinociception elicited by GABAergic tonic inhibition impairment in the posterior hypothalamus. European Neuropsychopharmacology, v. 27, n. 11, p. 1120-1131, NOV 2017. (07/01174-1, 17/11855-8, 12/03798-0)

FERREIRA-SGOBBI, RENATA; FIGUEIREDO, REBECA MACHADO; FRIAS, ALANA TERCINO; MATTHIESEN, MELINA; BATISTELA, MATHEUS FITIPALDI; FALCONI-SOBRINHO, LUIZ LUCIANO; VILELA-COSTA, HELOISA HELENA; SA, SUSANA ISABEL; LOVICK, THELMA ANDERSON; ZANGROSSI JR, HELIO; et al. Panic-like responses of female Wistar rats confronted by Bothrops alternatus pit vipers, or exposure to acute hypoxia: Effect of oestrous cycle. European Journal of Neuroscience, v. 55, n. 1, DEC 2021. (12/03798-0, 17/11855-8, 07/01174-1)

DOS ANJOS-GARCIA, TAYLION; COIMBRA, NORBERTO CYSNE. Opposing roles of dorsomedial hypothalamic CB1 and TRPV1 receptors in anandamide signaling during the panic-like response elicited in mice by Brazilian rainbow Boidae snakes. Psychopharmacology, v. 236, n. 6, p. 1863-1874, JUN 2019. (17/11855-8, 17/22647-7, 12/03798-0)

DE CARVALHO, MILENE CRISTINA; DE FIGUEIREDO, REBECA MACHADO; COIMBRA, NORBERTO CYSNE; ANDRADE LEITE-PANISSI, CHRISTIE RAMOS; DE SOUZA SILVA, MARIA ANGELICA; HUSTON, JOSEPH P.; MATTERN, CLAUDIA; BRANDAO, MARCUS LIRA. Intranasal dopamine attenuates fear responses induced by electric shock to the foot and by electrical stimulation of the dorsal periaqueductal gray matter. JOURNAL OF PSYCHOPHARMACOLOGY, v. 33, n. 12, JULY 2019. (17/11855-8)

PASCHOALIN-MAURIN, TATIANA. Repeated exposure of naive and peripheral nerve-injured mice to a snake as an experimental model of post-traumatic stress disorder and its co-morbidity with neuropathic pain. Brain Research, v. 1744, OCT 1 2020. (17/11855-8, 07/01174-1, 13/13398-2, 12/03798-0)

ALMADA, RAFAEL C.; FALCONI-SOBRINHO, LUIZ LUCIANO; DA SILVA, JULIANA A.; WOTJAK, CARSTEN T.; COIMBRA, NORBERTO C.. Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers. Psychopharmacology, v. N/A, p. 17-pg., 2022-06-02. (17/11855-8, 07/01174-1, 20/15050-7, 12/03798-0)

DOS SANTOS SAMPAIO, MARIA DE FATIMA; GIRALDI-GUIMARAES, ARTHUR; LOURENCO, CAMILA DA SILVA; PEREIRA, MESSIAS GONZAGA; COIMBRA, NORBERTO CYSNE. Effects of bone marrow mononuclear cells on induction of axonal sprouting in cortico-cortical and cortico-striatal pathways in an animal model of cortical ablation. BMC RESEARCH NOTES, v. 13, n. 1, JUN 3 2020. (17/11855-8)

ALMADA, RAFAEL CARVALHO; DOS ANJOS-GARCIA, TAYLLON; DA SILVA, JULIANA ALMEIDA; PIGATTO, GLAUCE REGINA; WOTJAK, CARSTEN T.; COIMBRA, NORBERTO CYSNE. The modulation of striatonigral and nigrotectal pathways by CB1 signalling in the substantia nigra pars reticulata regulates panic elicited in mice by urutu-cruzeiro lancehead pit vipers. Behavioural Brain Research, v. 401, MAR 5 2021. (07/01174-1, 12/03798-0, 17/11855-8)

DA SILVA, JULIANA ALMEIDA; ALMADA, RAFAEL CARVALHO; DE PAIVA, YARA BEZERRA; COIMBRA, NORBERTO CYSNE. Endocannabinoid neuromodulation in the neostriatum decreases the GABAergic striato-nigral disinhibitory function and increases the nigro-collicular inhibitory pathway activity. JOURNAL OF NEURAL TRANSMISSION, v. 127, n. 8, JUN 2020. (19/01713-7, 17/11855-8, 15/10313-1, 09/02458-9, 07/01174-1, 12/03798-0, 18/03898-1, 12/22681-7)

DOS ANJOS-GARCIA, TAYLLON; KANASHIRO, ALEXANDRE; DE CAMPOS, ALLINE CRISTINA; COIMBRA, NORBERTO CYSNE. Environmental Enrichment Facilitates Anxiety in Conflict-Based Tests but Inhibits Predator Threat-Induced Defensive Behaviour in Male Mice. NEUROPSYCHOBIOLOGY, v. N/A, p. 12-pg., 2022-01-13. (17/11855-8, 07/01174-1, 20/15050-7, 12/03798-0)

DE PAULA RODRIGUES, BRUNO MANGILI; COIMBRA, NORBERTO CYSNE. CB1 receptor signalling mediates cannabidiol-induced panicolytic-like effects and defensive antinociception impairment in mice threatened by Bothrops jararaca lancehead pit vipers. JOURNAL OF PSYCHOPHARMACOLOGY, v. 36, n. 12, p. 13-pg., 2022-08-10. (12/03798-0, 17/11855-8, 20/15050-7)

CALVO, FABRICIO; LOBAO-SOARES, BRUNO; DE FREITAS, RENATO LEONARDO; PASCHOALIN-MAURIN, TATIANA; DOS ANJOS-GARCIA, TAYLLON; MEDEIROS, PRISCILA; DA SILVA, JULIANA ALMEIDA; LOVICK, THELMA ANDERSON; COIMBRA, NORBERTO CYSNE. The endogenous opioid system modulates defensive behavior evoked by Crotalus durissus terrificus: Panicolytic-like effect of intracollicular non-selective opioid receptors blockade. JOURNAL OF PSYCHOPHARMACOLOGY, v. 33, n. 1, p. 51-61, JAN 2019. (14/11869-0, 07/01174-1, 15/10313-1, 17/11855-8, 09/02458-9, 12/03798-0, 13/12916-0, 12/25167-2)

CALVO, FABRICIO; ALMADA, RAFAEL CARVALHO; DOS ANJOS-GARCIA, TAYLLON; FALCONI-SOBRINHO, LUIZ LUCIANO; PASCHOALIN-MAURIN, TATIANA; BAZAGLIA-DE-SOUSA, GUILHERME; MEDEIROS, PRISCILA; DA SILVA, JULIANA ALMEIDA; LOBAO-SOARES, BRUNO; COIMBRA, NORBERTO CYSNE. Panicolytic-like effect of mu(1)-opioid receptor blockade in the inferior colliculus of prey threatened by Crotalus durissus terrificus pit vipers. JOURNAL OF PSYCHOPHARMACOLOGY, v. 33, n. 5, p. 577-588, MAY 2019. (09/02458-9, 13/10984-8, 17/11855-8, 17/13560-5, 12/25167-2, 12/03798-0, 07/01174-1, 15/10313-1, 12/22681-7)

MATIAS, JR., IVAIR; ELIAS, DAOUD HIBRAHIM; BERNARDINO GARCIA, CAMILA ARAUJO; SILVA, GUILHERME HENRIQUE; MEJIA, JORGE; CABRAL, FRANCISCO ROMERO; CAMARGO MIRANDA, ANA CLAUDIA; DA SILVA, SERGIO GOMES; LOPES, LUIZA DA SILVA; COIMBRA, NORBERTO CYSNE; et al. A new model of experimental hemispherotomy in young adult Rattus norvegicus: a neural tract tracing and SPECT in vivo study. JOURNAL OF NEUROSURGERY, v. 130, n. 4, p. 1210-1223, APR 2019. (07/01174-1, 17/11855-8, 12/03798-0)

PIGATTO, GLAUCE R.; QUINTEIRO, MAIARA H. S.; NUNES-DE-SOUZA, RICARDO L.; COIMBRA, NORBERTO C.; PARIZOTTO, NIVALDO A.. Low-Intensity Photobiomodulation Decreases Neuropathic Pain in Paw Ischemia-Reperfusion and Spared Nervus Ischiadicus Injury Experimental Models. PAIN PRACTICE, v. 20, n. 4, DEC 2019. (17/11855-8, 16/13941-6)

KHAN, ASMAT ULLAH; FALCONI-SOBRINHO, LUIZ LUCIANO; DOS ANJOS-GARCIA, TAYLLON; SANTOS, MARIA DE FATIMA DOS; CRIPPA, JOSE ALEXANDRE DE SOUZA; MENESCAL-DE-OLIVEIRA, LEDA; COIMBRA, NORBERTO CYSNE. Cannabidiol-induced panicolytic-like effects and fear-induced antinociception impairment: the role of the CB1 receptor in the ventromedial hypothalamus. Psychopharmacology, v. 237, n. 4, p. 1063-1079, APR 2020. (17/11855-8)

MENDES-GOMES, JOYCE; MOTTA, SIMONE CRISTINA; BINDI, RICARDO PASSONI; DE OLIVEIRA, AMANDA RIBEIRO; ULLAH, FARHAD; BALDO, MARCUS VINICIUS C.; COIMBRA, NORBERTO CYSNE; CANTERAS, NEWTON SABINO; BLANCHARD, D. CAROLINE. Defensive behaviors and brain regional activation changes in rats confronting a snake. Behavioural Brain Research, v. 381, MAR 2 2020. (17/11855-8, 14/05432-9, 13/13398-2, 18/00576-3)

DA SILVA, JULIANA ALMEIDA; BIAGIONI, AUDREY FRANCESCHI; ALMADA, RAFAEL CARVALHO; DE FREITAS, RENATO LEONARDO; COIMBRA, NORBERTO CYSNE. Panicolytic-like effects caused by substantia nigra pars reticulata pretreatment with low doses of endomorphin-1 and high doses of CTOP or the NOP receptors antagonist JTC-801 in male Rattus norvegicus. Psychopharmacology, v. 234, n. 20, p. 3009-3025, OCT 2017. (14/11869-0, 09/02458-9, 14/10742-7, 17/11855-8, 12/03798-0, 09/54014-7, 10/15140-4, 13/12916-0, 09/17258-5, 07/01174-1, 15/10313-1, 12/22681-7)

DE PAULA, B. B.; VIEIRA-RASTELI, E. B.; CALVO, F.; COIMBRA, N. C.; LEITE-PANISSI, C. R. A.. unctional activation of the periaqueductal gray matter during conditioned and unconditioned fear in guinea pigs confronted with the Boa constrictor constrictor snak. Brazilian Journal of Medical and Biological Research, v. 55, n. 1, 2022. (12/03798-0, 07/06549-3, 17/11855-8, 05/00096-1)

DOS SANTOS SAMPAIO, MARIA DE FATIMA; BASTOS BOECHAT, MARCELA SANTANA; GUSMAN CUNHA, IGOR AUGUSTO; PEREIRA, MESSIAS GONZAGA; COIMBRA, NORBERTO CYSNE; GIRALDI-GUIMARAES, ARTHUR. Neurotrophin-3 upregulation associated with intravenous transplantation of bone marrow mononuclear cells induces axonal sprouting and motor functional recovery in the long term after neocortical ischaemia. Brain Research, v. 1758, MAY 1 2021. (17/11855-8)

DOS ANJOS-GARCIA, TAYLLON; COIMBRA, NORBERTO CYSNE. Anandamide in the anterior hypothalamus diminishes defensive responses elicited in mice threatened by Epicrates cenchria constrictor serpents. ACTA NEUROBIOLOGIAE EXPERIMENTALIS, v. 80, n. 2, p. 179-191, 2020. (17/11855-8, 07/01174-1, 12/03798-0, 17/22647-7)

DE FIGUEIREDO, REBECA MACHADO; FALCONI-SOBRINHO, LUIZ LUCIANO; ANDRADE LEITE-PANISSI, CHRISTIE RAMOS; HUSTON, JOSEPH P.; MATTERN, CLAUDIA; DE CARVALHO, MILENE CRISTINA; COIMBRA, NORBERTO CYSNE. D-2-like receptor activation by intranasal dopamine attenuates fear responses induced by electrical stimulation of the dorsal periaqueductal grey matter, but fails to reduce aversion to pit vipers and T-maze performance. JOURNAL OF PSYCHOPHARMACOLOGY, v. 36, n. 11, p. 16-pg., 2022-10-14. (17/11855-8, 13/10984-8, 19/05255-3, 20/15050-7)

FALCONI-SOBRINHO, LUIZ LUCIANO; DOS ANJOS-GARCIA, TAYLLON; HERNANDES, PALOMA MOLINA; RODRIGUES, BRUNO MANGILI DE PAULA; ALMADA, RAFAEL CARVALHO; COIMBRA, NORBERTO CYSNE. Unravelling the dorsal periaqueductal grey matter NMDA receptors relevance in the nitric oxide-mediated panic-like behaviour and defensive antinociception organised by the anterior hypothalamus of male mice. Psychopharmacology, v. N/A, p. 17-pg., 2023-01-17. (17/22647-7, 12/22681-7, 17/11855-8, 07/01174-1, 19/01713-7, 18/03898-1, 13/10984-8, 20/15050-7, 12/03798-0)