Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens

Autor(es)

Abrao, Fariza ^[1] ; Silva, Thayna Souza ^[1] ; Moura, Claudia L. ^[1] ; Ambrosio, Sergio Ricardo ^[2] ; Sola Veneziani, Rodrigo Cassio ^[2] ; de Paiva, Raphael E. F. ^[3] ; Bastos, Jairo Kenupp ^[4] ; Gomes Martins, Carlos Henrique ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Franca, Res Lab Appl Microbiol, Franca, SP - Brazil

^[2] Univ Franca, Nucleus Res Sci & Technol, Franca, SP - Brazil

^[3] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[5] Univ Fed Uberlandia, Inst Biomed Sci ICBIM, Lab Antimicrobial Testing, Av Para 1720, BR-38405320 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 MAR 2 2021.
• Citações Web of Science: 0

Resumo

Invasion of periodontal tissues by Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can be associated with aggressive forms of periodontitis. Oleoresins from different copaifera species and their compounds display various pharmacological properties. The present study evaluates the antibacterial and antivirulence activity of oleoresins obtained from different copaifera species and of ten isolated compounds against two causative agents of periodontitis. The following assays were performed: determination of the minimum inhibitory concentration (MIC), determination of the minimum bactericidal concentration (MBC), and determination of the antibiofilm activity by inhibition of biofilm formation and biofilm eradication tests. The antivirulence activity was assessed by hemagglutination, P. gingivalis Arg-X and Lis-X cysteine protease inhibition assay, and A. actinomycetemcomitans leukotoxin inhibition assay. The MIC and MBC of the oleoresins and isolated compounds 1, 2, and 3 ranged from 1.59 to 50 mu g/mL against P. gingivalis (ATCC 33277) and clinical isolates and from 6.25 to 400 mu g/mL against A. actinomycetemcomitans (ATCC 43717) and clinical isolates. About the antibiofilm activity, the oleoresins and isolated compounds 1, 2, and 3 inhibited biofilm formation by at least 50% and eradicated pre-formed P. gingivalis and A. actinomycetemcomitans biofilms in the monospecies and multispecies modes. A promising activity concerning cysteine protease and leucotoxin inhibition was also evident. In addition, molecular docking analysis was performed. The investigated oleoresins and their compounds may play an important role in the search for novel sources of agents that can act against periodontal pathogens. (AU)

• Processo FAPESP: 18/21537-6 - Aplicações de ligantes imínicos polidentados na modulação da reatividade de íons cobre em doenças relacionadas a inflamação e em reações de acoplamento bio-ortogonais
• Beneficiário: Raphael Enoque Ferraz de Paiva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/23012-2 - Potencial antibacteriano e antivirulência do óleo-resina e substâncias isoladas de espécies de Copaifera spp. frente aos principais agentes periodontais
• Beneficiário: Sergio Ricardo Ambrosio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático