Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains

Autor(es)

Machado, Francielly Cristina ^[1] ; Bittar, Cintia ^[1] ; Rahal, Paula ^[1] ; Calmon, Marilia Freitas ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Inst Biosci Humanities & Exact Sci, Dept Biol, IBILCE, Rua Cristovao Colombo 2265, Bairro Jardim Nazareth, BR-15054010 Sao Jose De Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 166, n. 6 APR 2021.
• Citações Web of Science: 0

Resumo

Infection with distinct Zika virus (ZIKV) strains in in vitro and in vivo models has demonstrated that the host's response to infection is strain-dependent. There has been no analysis of the impact of infection with different ZIKV strains on miRNA expression in human cells. We investigated miRNA expression in PNT1A cells upon infection with an African ZIKV strain (MR766) and a Brazilian ZIKV strain (ZIKV(BR)) using PCR array. Sixteen miRNAs were modulated in PNT1A cells: six miRNAs were modulated by both strains, while a set of ten miRNAs were modulated exclusively by ZIKV(BR) infection. In silico analysis showed that nine significant KEGG pathways and eight significant GO terms were predicted to be enriched upon ZIKV(BR) infection, and these pathways were related to cancer, environmental information processing, metabolism, and extracellular matrix. Differential modulation of miRNA expression suggests that distinct strains of ZIKV can differentially modulate the host response through the action of miRNAs. (AU)

• Processo FAPESP: 17/09197-2 - Análise da expressão de miRNAs em células humanas de próstata infectadas com o Zika vírus
• Beneficiário: Francielly Cristina Machado
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/22198-0 - Potencial terapêutico de fármacos flavonóidicos na infecção com o vírus da hepatite C
• Beneficiário: Paula Rahal
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/16660-5 - Estudo de nanoemulsões associadas a terapia fotodinâmica no tratamento do câncer de mama
• Beneficiário: Marilia de Freitas Calmon
• Modalidade de apoio: Auxílio à Pesquisa - Regular