Polyols-related gene expression is affected by cyclic desiccation in lichen microalgae

Autor(es)

Hell, Aline F. ^{[1, 2]} ; Gasulla, Francisco ^[2] ; Gonzalez-Houcarde, Maria ^[2] ; Pelegrino, Milena T. ^[1] ; Seabra, Amedea B. ^[1] ; del Campo, Eva M. ^[2] ; Casano, Leonardo M. ^[2] ; Centeno, Danilo C. ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ ABC, Ctr Nat Sci & Humanities, Sao Bernardo Do Campo, SP - Brazil

^[2] Univ Alcala De Henares, Dept Life Sci, Alcala De Henares, Madrid - Spain

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL AND EXPERIMENTAL BOTANY; v. 185, MAY 2021.
• Citações Web of Science: 1

Resumo

The accumulation of compatible solutes, as sugar and polyols, as a response to stress is a widely biochemical adaptation. However, the signaling process involved in this regulation remains unclear. Lichens and their microalgae have to cope with a variety of stresses associated with sudden changes in water content during daily cycles of desiccation-rehydration (D/R). In this way, we hypothesized: (1) the differences in desiccation tolerance among lichen-forming microalgae could be associated with species-specific differences in their polyol metabolism and (2) the nitric oxide (NO), as a signaling molecule, could assist the regulation of the expression of polyol-related genes in these organisms. Therefore, we carried out a comparative analysis of the transcriptional expression of key genes involved in the synthesis of polyols and metabolite levels, along with the cellular concentration of NO in isolated lichen microalgae during consecutive daily cycles of D/R and correlated the results with the polyol profile and NO measurements. Two microalgae were employed, Trebouxia sp. TR9 (TR9) and Coccomyxa simplex (Csol), obtained from lichens subjected to different water availability in their natural habitat. Our results revealed that, in both microalgae, the expression of myo-inositol and raffinose-related genes is modulated by cyclic D/R. Although, genes seem more prone to be transcriptionally upregulated by cyclic D/R in Csol than in TR9, indicating a more inducible defensive response in Csol in contrast with a constitutive defense system in TR9. Correlation analysis provided evidences that NO may act as a signaling molecule, through the modulation of polyol biosynthetic pathway and may be related to a primed memory state, by assisting in the rapidly and more precise metabolic response to subsequent D/R cycles. (AU)

• Processo FAPESP: 18/15576-9 - Metabolismo do carbono em plantas sob diferentes perspectivas: buscando alternativas para aumento de produtividade
• Beneficiário: Danilo da Cruz Centeno
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 18/08194-2 - Óleo essencial contendo nanopartículas metálicas funcionalizadas com óxido nítrico como estratégia para o controle de patógenos vegetais na agricultura
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular