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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polyols-related gene expression is affected by cyclic desiccation in lichen microalgae

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Author(s):
Hell, Aline F. [1, 2] ; Gasulla, Francisco [2] ; Gonzalez-Houcarde, Maria [2] ; Pelegrino, Milena T. [1] ; Seabra, Amedea B. [1] ; del Campo, Eva M. [2] ; Casano, Leonardo M. [2] ; Centeno, Danilo C. [1]
Total Authors: 8
Affiliation:
[1] Fed Univ ABC, Ctr Nat Sci & Humanities, Sao Bernardo Do Campo, SP - Brazil
[2] Univ Alcala De Henares, Dept Life Sci, Alcala De Henares, Madrid - Spain
Total Affiliations: 2
Document type: Journal article
Source: ENVIRONMENTAL AND EXPERIMENTAL BOTANY; v. 185, MAY 2021.
Web of Science Citations: 1
Abstract

The accumulation of compatible solutes, as sugar and polyols, as a response to stress is a widely biochemical adaptation. However, the signaling process involved in this regulation remains unclear. Lichens and their microalgae have to cope with a variety of stresses associated with sudden changes in water content during daily cycles of desiccation-rehydration (D/R). In this way, we hypothesized: (1) the differences in desiccation tolerance among lichen-forming microalgae could be associated with species-specific differences in their polyol metabolism and (2) the nitric oxide (NO), as a signaling molecule, could assist the regulation of the expression of polyol-related genes in these organisms. Therefore, we carried out a comparative analysis of the transcriptional expression of key genes involved in the synthesis of polyols and metabolite levels, along with the cellular concentration of NO in isolated lichen microalgae during consecutive daily cycles of D/R and correlated the results with the polyol profile and NO measurements. Two microalgae were employed, Trebouxia sp. TR9 (TR9) and Coccomyxa simplex (Csol), obtained from lichens subjected to different water availability in their natural habitat. Our results revealed that, in both microalgae, the expression of myo-inositol and raffinose-related genes is modulated by cyclic D/R. Although, genes seem more prone to be transcriptionally upregulated by cyclic D/R in Csol than in TR9, indicating a more inducible defensive response in Csol in contrast with a constitutive defense system in TR9. Correlation analysis provided evidences that NO may act as a signaling molecule, through the modulation of polyol biosynthetic pathway and may be related to a primed memory state, by assisting in the rapidly and more precise metabolic response to subsequent D/R cycles. (AU)

FAPESP's process: 18/15576-9 - Carbon metabolism in plants under different perspectives: searching alternatives for crop yield enhancement
Grantee:Danilo da Cruz Centeno
Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
Grantee:Amedea Barozzi Seabra
Support Opportunities: Regular Research Grants