Rosmarinus officinalis L. (rosemary) extract has antibiofilm effect similar to the antifungal nystatin on Candida samples

Autor(es)

Meccatti, Vanessa M. ^[1] ; De Oliveira, Jonatas R. ^[2] ; Figueira, Leandro W. ^[1] ; Netto, Amandio A. Lagareiro ^[3] ; Zamarioli, Lucas S. ^[4] ; Marcucci, Maria C. ^[1] ; Camargo, Samira E. A. ^[5] ; Carvalho, Claudio A. T. ^[6] ; De Oliveira, Luciane D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Ciencia & Tecnol, Dept Biociencias & Diagnost Bucal, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Anhembi Morumbi, Escola Med, Av Dep Benedito Matarazzo 4050, BR-12230002 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Anhanguera, Dept Farm, Av Raimundo Pereira de Magalhaes 3305, BR-05145200 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Inst Farmacol & Biol Mol, Dept Modo Acao Drogas, Rua Tres de Maio 100, BR-04044020 Sao Paulo, SP - Brazil

^[5] Univ Florida, Coll Dent, Dept Restorat Dent Sci, Gainesville, FL 32610 - USA

^[6] Univ Estadual Paulista UNESP, Inst Ciencia & Tecnol, Dept Odontol Restauradora, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anais da Academia Brasileira de Ciências; v. 93, n. 2 2021.
• Citações Web of Science: 0

Resumo

Abstract Candida spp. are naturally opportunistic and can promote infections. These yeasts can form biofilm, after penetration and adhesion to the biotic or abiotic surfaces. Preexisting diseases, treatments with drugs and radiation therapy, medical procedures, and parafunctional habits favor the installation of a fungal infection. Increased resistance to the available antifungals has become a concern. Therefore, alternative methods to control them have been evaluated, including the use of plant substances. In this study, the antibiofilm effect of R. officinalis L. extract was analyzed on C. albicans, C. dubliniensis, C. glabrata, C. krusei, and C. tropicalis. A phytochemical analysis of the extract was performed. Biofilms were formed for 48 h and exposed to the different concentrations of the extract (50, 100, and 200 mg/mL) for 5 min or 24 h. The effect of the plant extract was compared to the antifungal nystatin. Rosmarinus officinalis L. extract was constituted of phenols and flavonoids, highlighting the presence of chlorogenic acid derivatives in its composition. Biofilm reductions were observed after exposure to the plant extract for both periods. The plant extract provided a reduction similar to the antifungal. Thus, R. officinalis L. extract showed antibiofilm effect on Candida spp. comparable to the nystatin. (AU)

• Processo FAPESP: 15/25338-0 - Atividade antifúngica e anti-inflamatória do extrato glicólico de Rosmarinus officinalis L. (alecrim)
• Beneficiário: Vanessa Marques Meccatti Domiciano
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/08776-3 - Atividade antimicrobiana, anti-inflamatória, citotoxicidade e genotoxicidade de diferentes extratos naturais
• Beneficiário: Luciane Dias de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular