Response of pulp cells to resin infiltration of enamel white spot-like lesions

Autor(es)

Mendes Soares, Igor Paulino ^[1] ; Anovazzi, Giovana ^[2] ; Anselmi, Caroline ^[2] ; Leite, Maria Luisa ^[1] ; Salles Scheffel, Debora Lopes ^[3] ; Soares, Diana Gabriela ^[4] ; de Souza Costa, Carlos Alberto ^[5] ; Hebling, Josimeri ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Morphol Genet Orthodont & Pediat Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[3] State Univ Maringa UEM, Sch Dent, Dept Dent, Maringa, Parana - Brazil

^[4] Sao Paulo Univ USP, Bauru Fac Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 37, n. 6, p. E329-E340, JUN 2021.
• Citações Web of Science: 1

Resumo

Objectives. To investigate the trans-enamel and trans-dentinal biological effects of treating enamel white spot-like lesions (EWSLs) with resin infiltration components (RICs) on odontoblast-like cells (MDPC-23) and human dental pulp cells (HDPCs). Methods. EWSLs were induced in 60 enamel/dentin discs (4.0 +/- 0.2 mm thick) using S. mutans. The discs were adapted into artificial pulp chambers and MDPC-23 were seeded on the dentin surface. The components of a resin infiltration system (Icon) were applied individually or in combination on the enamel surface as following (n = 10/treatment): Etch, Infiltrant, Etch+Infiltrant, or Etch+Dry+Infiltrant. The application of water or hydrogen peroxide served as negative and positive controls, respectively. After 72 h, MDPC-23 viability was evaluated. The extracts were exposed for 72 h to pre-cultured MDPC-23 and HDPCs in 96-well plates to evaluate cell viability, alkaline phosphatase activity (ALP), mineralized nodule formation (MN), and the expression of inflammatory cytokines (ICs) and mineralization-related genes (MRs). Data were analyzed by ANOVA complemented with Tukey or Games-Howell post-hocs (a = 5%). Results. Cell viability, ALP activity, and MN formation were significantly reduced in response to the RICs, presenting intermediate values compared to positive and negative controls. Likewise, ICs were upregulated, whereas MRs were downregulated. Among the RICs, the Etch component caused the most notorious detrimental effects. (AU)

• Processo FAPESP: 18/04867-2 - Citotoxicidade trans-amelodentinária de um infiltrante resinoso de cárie sobre células pulpares
• Beneficiário: Igor Paulino Mendes Soares
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica