Cryo-EM structure of the mature and infective Mayaro virus at 4.4 angstrom resolution reveals features of arthritogenic alphaviruses

Autor(es):Mostrar menos -

Ribeiro-Filho, V, Helder ; Coimbra, Lais D. ^[1] ; Cassago, Alexandre ^[2] ; Rocha, Rebeca P. F. ^[1] ; da Silva Guerra, Joao Victor ^[1] ; de Felicio, Rafael ^[1] ; Carnieli, Carolina Moretto ^[1] ; Leme, Luiza ^[1] ; Padilha, Antonio Claudio ^[2] ; Paes Leme, Adriana F. ^[1] ; Trivella, Daniela B. B. ^[1] ; Portugal, Rodrigo Villares ^[2] ; Lopes-de-Oliveira, Paulo Sergio ^[1] ; Marques, Rafael Elias ^[1]

Afiliação do(s) autor(es)

^[1] Ribeiro-Filho, Helder, V, Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab, Campinas, SP - Brazil

^[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NATURE COMMUNICATIONS; v. 12, n. 1 MAY 24 2021.
• Citações Web of Science: 2

Resumo

Mayaro virus (MAYV) is an emerging arbovirus of the Americas that may cause a debilitating arthritogenic disease. The biology of MAYV is not fully understood and largely inferred from related arthritogenic alphaviruses. Here, we present the structure of MAYV at 4.4 angstrom resolution, obtained from a preparation of mature, infective virions. MAYV presents typical alphavirus features and organization. Interactions between viral proteins that lead to particle formation are described together with a hydrophobic pocket formed between E1 and E2 spike proteins and conformational epitopes specific of MAYV. We also describe MAYV glycosylation residues in E1 and E2 that may affect MXRA8 host receptor binding, and a molecular ``handshake{''} between MAYV spikes formed by N262 glycosylation in adjacent E2 proteins. The structure of MAYV is suggestive of structural and functional complexity among alphaviruses, which may be targeted for specificity or antiviral activity. Mayaro virus (MAYV) is an emerging arbovirus in Central and South America that is transmitted by mosquitoes and causes arthritogenic disease. Here, the authors present the 4.4 angstrom resolution cryo-EM structure of MAYV and describe specific features of the virus, which could be exploited for the design of MAYV-specific diagnostics and therapeutics. (AU)

• Processo FAPESP: 18/00629-0 - Detecção e caracterização de cavidades proteicas através de computação paralela e descritores moleculares
• Beneficiário: Paulo Sergio Lopes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/03917-6 - Mecanismos de doença e resistência envolvidos na febre do Mayaro em camundongos
• Beneficiário: Rafael Elias Marques Pereira Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/15340-2 - EMU: aquisição de microscópio eletrônico de transmissão para criomicroscopia eletrônica de partículas isoladas - estabelecimento de uma instalação aberta de criomicroscopia eletrônica no CNPEM
• Beneficiário: Rodrigo Villares Portugal
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários