| Full text | |
| Author(s): Show less - |
Ribeiro-Filho, V, Helder
;
Coimbra, Lais D.
[1]
;
Cassago, Alexandre
[2]
;
Rocha, Rebeca P. F.
[1]
;
da Silva Guerra, Joao Victor
[1]
;
de Felicio, Rafael
[1]
;
Carnieli, Carolina Moretto
[1]
;
Leme, Luiza
[1]
;
Padilha, Antonio Claudio
[2]
;
Paes Leme, Adriana F.
[1]
;
Trivella, Daniela B. B.
[1]
;
Portugal, Rodrigo Villares
[2]
;
Lopes-de-Oliveira, Paulo Sergio
[1]
;
Marques, Rafael Elias
[1]
Total Authors: 14
|
| Affiliation: | [1] Ribeiro-Filho, Helder, V, Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab, Campinas, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab, Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | NATURE COMMUNICATIONS; v. 12, n. 1 MAY 24 2021. |
| Web of Science Citations: | 2 |
| Abstract | |
Mayaro virus (MAYV) is an emerging arbovirus of the Americas that may cause a debilitating arthritogenic disease. The biology of MAYV is not fully understood and largely inferred from related arthritogenic alphaviruses. Here, we present the structure of MAYV at 4.4 angstrom resolution, obtained from a preparation of mature, infective virions. MAYV presents typical alphavirus features and organization. Interactions between viral proteins that lead to particle formation are described together with a hydrophobic pocket formed between E1 and E2 spike proteins and conformational epitopes specific of MAYV. We also describe MAYV glycosylation residues in E1 and E2 that may affect MXRA8 host receptor binding, and a molecular ``handshake{''} between MAYV spikes formed by N262 glycosylation in adjacent E2 proteins. The structure of MAYV is suggestive of structural and functional complexity among alphaviruses, which may be targeted for specificity or antiviral activity. Mayaro virus (MAYV) is an emerging arbovirus in Central and South America that is transmitted by mosquitoes and causes arthritogenic disease. Here, the authors present the 4.4 angstrom resolution cryo-EM structure of MAYV and describe specific features of the virus, which could be exploited for the design of MAYV-specific diagnostics and therapeutics. (AU) | |
| FAPESP's process: | 18/00629-0 - Detection and characterization of protein cavities using parallel computing and molecular descriptors |
| Grantee: | Paulo Sergio Lopes de Oliveira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/03917-6 - Mechanisms of disease susceptibility and resistance in experimental Mayaro fever in mice |
| Grantee: | Rafael Elias Marques Pereira Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/15340-2 - EMU: acquisition of a transmission electron microscope for single particle cryo electron microscopy - establishing a cryo electron microscopy open facility at CNPEM |
| Grantee: | Rodrigo Villares Portugal |
| Support Opportunities: | Multi-user Equipment Program |