Statherin-derived peptides as antifungal strategy against Candida albicans

Autor(es)

Guimaraes Pellissari, Claudia Viviane ^{[1, 2]} ; Jorge, Janaina Habib ^[1] ; Marin, Lina Maria ^[2] ; Sabino-Silva, Robinson ^[3] ; Siqueira, Walter Luiz ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, UNESP, 1680 Rua Humaita, BR-14801385 Araraquara, SP - Brazil

^[2] Univ Saskatchewan, Coll Dent, 105 Wiggins Rd, Saskatoon, SK S7N 5E4 - Canada

^[3] Univ Fed Uberlandia, Inst Biomed Sci, Dept Physiol, 3Q-2121 Av Joao Naves de Avila, BR-38400902 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 125, MAY 2021.
• Citações Web of Science: 0

Resumo

Objective: The aim of this in vitro study was to evaluate the effect of statherin and its naturally occurring peptides (DR9-2, DR9, GE-12, IT-32, GQ-19, IP-18) on Candida albicans metabolism and biofilm development. Design: After the killing assay, a peptide pellicle was formed on the bottom of a polystyrene plate at the IC50 of each peptide. Over the peptide pellicle, Candida albicans biofilm (48 h) was grown. The peptides antimicrobial activity after the peptides treatment was evaluated by alamarBlue, total biofilm biomass and colony forming units (CFU) counting. Results: The pellicle with statherin and the peptides (DR9-2, DR9, GE-12, IP-18, GQ-19) was able to reduce he viability of Candida albicans compared to the negative control. They also decreased cell proliferation by 20 % and total biomass. IT-32 showed the highest reduction in cell proliferation and biomass, which was similar to the positive control, histatin 5. Conclusions: These results suggest that the naturally occuring peptides from statherin are able to decrease Candida albicans colonization and biofilm proliferation. (AU)

• Processo FAPESP: 16/03847-2 - Atividade antifúngica e citotoxicidade, em co-cultura, de biomateriais revestidos com nanopartículas de quitosana contendo moléculas de proteínas salivares encapsuladas (histatina 1, histatina 5 ou DR9RR14)
• Beneficiário: Janaina Habib Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/06016-1 - Atividade antifúngica e citotoxicidade, em co-cultura, de biomateriais revestidos com nanopartículas de chitosan contendo moléculas de proteínas salivares, histatin 1, histatin 5 ou DR9RR14 encapsuladas
• Beneficiário: Cláudia Viviane Guimarães Pellissari
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/13904-9 - Avaliação de novos peptídeos salivares para tratamento da candidíase oral
• Beneficiário: Cláudia Viviane Guimarães Pellissari
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado