New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans

Autor(es)

Ungari, Leticia Pereira ^[1] ; Netherlands, Edward C. ^[2] ; Quagliatto Santos, Andre Luiz ^[3] ; de Alcantara, Edna Paulino ^[1] ; Emmerich, Enzo ^[1] ; da Silva, Reinaldo Jose ^[1] ; O'Dwyer, Lucia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Biociencias, DBBVPZ, Setor Parasitol, Botucatu, SP - Brazil

^[2] North West Univ, Unit Environm Sci & Management, Private Bag x6001, ZA-2520 Potchefstroom - South Africa

^[3] Univ Fed Uberlandia, Fac Med Vet, Lab Ensino & Pesquisa Anim Silvestres, Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE; v. 14, p. 190-201, APR 2021.
• Citações Web of Science: 0

Resumo

The Amphibia are considered the most threatened vertebrate class globally, yet in Brazil they are also one of the more diverse and species rich groups. Although, in recent years there has been strong focus on amphibian related research, their parasites have not received the same attention. In Brazil, only a single species of Hepatozoon, namely H. leptodactyli (Lesage, 1908) Pessoa, 1970, has been described from anuran hosts. The present study aimed to describe three new species of Hepatozoon parasitising Leptodactylus labyrinthicus and Leptodactylus latrans from Mato Grosso State, Brazil. From 66 anurans screened for haemogregarines, four belonging to the Leptodactylidae were found positive for species of Hepatozoon. Based on the morphological analysis of peripheral blood gamonts and spleen and liver tissue meronts, three different morphotypes of Hepatozoon spp. were identified. Morphotype 1 (M1) and morphotype 2 (M2) in L. labyrinthicus and morphotype 3 (M3) in L. latrans. Molecular data based on partial 18S rDNA sequences revealed an interspecific divergence, between the species ranging from 0.43% to 1.16%. Phylogenetic analysis recovered isolates from the present study monophyletic with other isolates from Brazilian reptile and anuran hosts, sister to a clade comprising species isolated from African, North American and European reptile and anuran host species. Thus, using morphological and molecular analysis three new species infecting Brazilian Leptodactylidae anurans were identified and described. This study increases the knowledge of Brazilian anurans blood parasites and demonstrates the importance of using integrative approaches for diagnosis of hemoparasites. (AU)

• Processo FAPESP: 18/00754-9 - Biodiversidade de hemoparasitos associados a anfíbios e répteis das regiões Centro-Oeste e Sudeste brasileiras
• Beneficiário: Letícia Pereira Úngari
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/09623-4 - Biodiversidade de parasitos associados a anfíbios e répteis das regiões centro-oeste e sudeste brasileiras
• Beneficiário: Lucia Helena O'Dwyer de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/50377-1 - Taxonomia morfológica e molecular de helmintos parasitas de animais ectotérmicos no Brasil
• Beneficiário: Reinaldo José da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular