Selective Extraction of Piceatannol from Passiflora edulis by-Products: Application of HSPs Strategy and Inhibition of Neurodegenerative Enzymes

Autor(es)

dos Santos, Luana Cristina ^{[1, 2]} ; Mendiola, Jose Antonio ^[2] ; Sanchez-Camargo, Andrea del Pilar ^[3] ; Alvarez-Rivera, Gerardo ^[2] ; Vigano, Juliane ^[4] ; Cifuentes, Alejandro ^[2] ; Ibanez, Elena ^[2] ; Martinez, Julian ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Engn & Technol, Lab High Pressure Food Engn LAPEA, R Monteiro Lobato 80, BR-13083862 Campinas - Brazil

^[2] CSIC, Lab Food, Inst Food Sci Res, CIAL, Nicolas Cabrera 9, Madrid 28049 - Spain

^[3] Univ Los Andes, Fac Engn, Dept Chem & Food Engn, Carrera 1 18A-12, Bogota 111711 - Colombia

^[4] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Chem Engn, R Sao Nicolau 210, BR-09913030 Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 12 JUN 2021.
• Citações Web of Science: 1

Resumo

Passiflora edulis by-products (PFBP) are a rich source of polyphenols, of which piceatannol has gained special attention recently. However, there are few studies involving environmentally safe methods for obtaining extracts rich in piceatannol. This work aimed to concentrate piceatannol from defatted PFBP (d-PFBP) by means of pressurized liquid extraction (PLE) and conventional extraction, using the bio-based solvents selected with the Hansen solubility parameters approach. The relative energy distance (Ra) between solvent and solute was: Benzyl Alcohol (BnOH) < Ethyl Acetate (EtOAc) < Ethanol (EtOH) < EtOH:H2O. Nonetheless, EtOH presented the best selectivity for piceatannol. Multi-cycle PLE at 110 degrees C was able to concentrate piceatannol 2.4 times more than conventional extraction. PLE exhibited a dependence on kinetic parameters and temperature, which could be associated with hydrogen bonding forces and the dielectric constant of the solvents. The acetylcholinesterase (AChE) and lipoxygenase (LOX) IC50 were 29.420 mu g/mL and 27.682 mu g/mL, respectively. The results reinforce the demand for processes to concentrate natural extracts from food by-products. (AU)

• Processo FAPESP: 17/23670-2 - Biorrefinaria para aproveitamento de subprodutos do processamento de maracujá com tecnologias de alta pressão
• Beneficiário: Julian Martínez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/11245-5 - Fracionamento de extratos de subprodutos industriais de maracujá-azedo (Passiflora edulis) por redução de pressão utilizando CO2 supercrítico
• Beneficiário: Luana Cristina dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/10655-0 - Bioatividade de extratos de sub-produtos de maracujá amarelo e sua eficiência na prevenção de doenças neurodegenerativas
• Beneficiário: Luana Cristina dos Santos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado