Selective Extraction of Piceatannol from Passiflora edulis by-Products: Application of HSPs Strategy and Inhibition of Neurodegenerative Enzymes

Author(s)

dos Santos, Luana Cristina ^{[1, 2]} ; Mendiola, Jose Antonio ^[2] ; Sanchez-Camargo, Andrea del Pilar ^[3] ; Alvarez-Rivera, Gerardo ^[2] ; Vigano, Juliane ^[4] ; Cifuentes, Alejandro ^[2] ; Ibanez, Elena ^[2] ; Martinez, Julian ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Engn & Technol, Lab High Pressure Food Engn LAPEA, R Monteiro Lobato 80, BR-13083862 Campinas - Brazil

^[2] CSIC, Lab Food, Inst Food Sci Res, CIAL, Nicolas Cabrera 9, Madrid 28049 - Spain

^[3] Univ Los Andes, Fac Engn, Dept Chem & Food Engn, Carrera 1 18A-12, Bogota 111711 - Colombia

^[4] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Chem Engn, R Sao Nicolau 210, BR-09913030 Diadema - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 12 JUN 2021.
• Web of Science Citations: 1

Abstract

Passiflora edulis by-products (PFBP) are a rich source of polyphenols, of which piceatannol has gained special attention recently. However, there are few studies involving environmentally safe methods for obtaining extracts rich in piceatannol. This work aimed to concentrate piceatannol from defatted PFBP (d-PFBP) by means of pressurized liquid extraction (PLE) and conventional extraction, using the bio-based solvents selected with the Hansen solubility parameters approach. The relative energy distance (Ra) between solvent and solute was: Benzyl Alcohol (BnOH) < Ethyl Acetate (EtOAc) < Ethanol (EtOH) < EtOH:H2O. Nonetheless, EtOH presented the best selectivity for piceatannol. Multi-cycle PLE at 110 degrees C was able to concentrate piceatannol 2.4 times more than conventional extraction. PLE exhibited a dependence on kinetic parameters and temperature, which could be associated with hydrogen bonding forces and the dielectric constant of the solvents. The acetylcholinesterase (AChE) and lipoxygenase (LOX) IC50 were 29.420 mu g/mL and 27.682 mu g/mL, respectively. The results reinforce the demand for processes to concentrate natural extracts from food by-products. (AU)

• FAPESP's process: 17/23670-2 - Biorefinery for the recovery of by-products from passion fruit processing through high pressure technologies
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/11245-5 - Fractionation of passion fruit (Passiflora edulis) by-products extracts by reducing pressure using supercritical CO2
• Grantee: Luana Cristina dos Santos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/10655-0 - Bioactivity of extracts from passion fruit by-products and their efficiency in preventing neurodegenerative diseases
• Grantee: Luana Cristina dos Santos
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate