Acai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line

Autor(es):Mostrar menos -

Neto da Silva, Marcos Antonio Custodio ^[1] ; Costa, Jonas Henrique ^[2] ; Pacheco-Fill, Taicia ^[2] ; Gois Ruiz, Ana Lucia Tasca ^[3] ; Branco Vidal, Flivia Castello ^[4] ; Assuncao Borges, Katia Regina ^[4] ; Araujo Guimaraes, Sulayne Janaina ^[5] ; Silva de Azevedo-Santos, Ana Paula ^[5] ; Buglio, Kaio Eduardo ^[3] ; Foglio, Mary Ann ^[3] ; Lacerda Barbosa, Maria do Carmo ^[6] ; Brandao Nascimento, Maria do Desterro Soares ^[4] ; de Carvalho, Joao Ernesto ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Postgrad Program Internal Med, Rua Tessalia Vieira de Camargo 126, BR-13083887 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

^[4] Fed Univ Maranhao UFMA, Dept Patol, Postgrad Program Adult Heath, BR-65080805 Sao Luis, Maranhao - Brazil

^[5] Fed Univ Maranhao UFMA, Postgrad Program Hlth Sicencies, BR-65080805 Sao Luis, Maranhao - Brazil

^[6] Fed Univ Maranhao UFMA, Dept Med 1, Postgrad Program Family Hlth, BR-65080805 Sao Luis, Maranhao - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 26, n. 12 JUN 2021.
• Citações Web of Science: 0

Resumo

Euterpe oleracea Mart. (acai) is a native palm from the Amazon region. There are various chemical constituents of acai with bioactive properties. This study aimed to evaluate the chemical composition and cytotoxic effects of acai seed extract on breast cancer cell line (MCF-7). Global Natural Products Social Molecular Networking (GNPS) was applied to identify chemical compounds present in acai seed extract. LC-MS/MS and molecular networking were employed to detect the phenolic compounds of acai. The antioxidant activity of acai seed extract was measured by DPPH assay. MCF-7 breast cancer cell line viability was evaluated by MTT assay. Cell death was evaluated by flow cytometry and time-lapse microscopy. Autophagy was evaluated by orange acridin immunofluorescence assay. Reactive oxygen species (ROS) production was evaluated by DAF assay. From the molecular networking, fifteen compounds were identified, mainly phenolic compounds. The acai seed extract showed cytotoxic effects against MCF-7, induced morphologic changes in the cell line by autophagy and increased the ROS production pathway. The present study suggests that acai seed extract has a high cytotoxic capacity and may induce autophagy by increasing ROS production in breast cancer. Apart from its antioxidant activity, flavonoids with high radical scavenging activity present in acai also generated NO (nitric oxide), contributing to its cytotoxic effect and autophagy induction. (AU)

• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático