Leishmanicidal Activity and Ultrastructural Changes of Maslinic Acid Isolated from Hyptidendron canum

Autor(es)

Adriana Jesus, Jessica ^[1] ; Laurenti, Marcia Dalastra ^[1] ; Lopes Silva, Matheus ^[2] ; Ghilardi Lago, Joao Henrique ^[2] ; Domingues Passero, Luiz Felipe ^{[3, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Med Sch, Dept Pathol, Lab Pathol Infect Dis LIM50, Av Dr Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[2] Fed Univ ABC UFABC, Ctr Nat & Human Sci, BR-09210580 Santo Andre, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Sao Vicente Praca Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Inst Adv Studies Ocean, Sao Vicente Joao Francisco Bensdorp 1178, BR-11350011 Sao Vicente, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; v. 2021, MAY 31 2021.
• Citações Web of Science: 0

Resumo

The therapeutic arsenal for the treatment of leishmaniasis is limited and has serious obstacles, such as variable activity, high toxicity, and costs. To overcome such limitations, it becomes urgent to characterize new bioactive molecules. Plants produce and accumulate different classes of bioactive compounds, and these molecules can be studied as a strategy to combat leishmaniasis. The study presented herein evaluated the leishmanicidal effect of maslinic acid isolated from the leaves of Hyptidendron canum (Lamiaceae) and investigated the morphological that occurred on Leishmania (Leishmania) infantum upon treatment. Maslinic acid was active and selective against promastigote and amastigote forms in a dose-dependent manner. Additionally, it was not toxic to peritoneal macrophages isolated from golden hamsters, while miltefosine and amphotericin B showed mild toxicity for macrophages. Morphological changes in prom astigotes of L. (L.) infantum treated with maslinic acid were related to cytoplasmic degeneration, intense exocytic activity, and blebbing in the kDNA; disruption of mitochondrial cristae was observed in some parasites. The nucleus of promastigote forms seems to be degraded and the chromatin fragmented, suggesting that maslinic acid triggers programmed cell death. These results indicate that maslinic acid may be an interesting molecule to develop new classes of drugs against leishmaniasis. (AU)

• Processo FAPESP: 16/10324-6 - Nanopartículas lípidicas como carreadores de triterpenos para o tratamento da Leishmaniose Visceral Experimental
• Beneficiário: Jéssica Adriana de Jesus
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/00468-0 - Uso do reposicionamento de fármacos e da bioprospecção de produtos naturais para caracterizar compostos com ação leishmanicida in vitro e in vivo
• Beneficiário: Luiz Felipe Domingues Passero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/02789-7 - Busca de metabólitos bioativos com ação antiparasitária em espécies vegetais de regiões de Mata Atlântica e Cerrado - uma abordagem química, fenotípica e metabolômica
• Beneficiário: João Henrique Ghilardi Lago
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 18/04080-2 - Preparação e caracterização físico química de Nanopartículas Lipídicas como carreadores de triterpenos para o tratamento da Leishmaniose Visceral.
• Beneficiário: Jéssica Adriana de Jesus
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado