In Vitro Susceptibility to Miltefosine of Leishmania infantum (syn. L. chagasi) Isolates from Different Geographical Areas in Brazil

Autor(es)

Espada, Caroline Ricce ^[1] ; Levatti, Erica V. de Castro ^[1] ; Boite, Mariana Cortes ^[2] ; Lamounier, Dorcas ^[3] ; Alvar, Jorge ^[4] ; Cupolillo, Elisa ^[2] ; Costa, Carlos Henrique Nery ^[3] ; Rode, Joelle ^[4] ; Uliana, Silvia R. B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508000 Sao Paulo - Brazil

^[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Pesquisa Leishmanioses, BR-21040900 Rio De Janeiro - Brazil

^[3] Univ Fed Piaui, Hosp Doencas Trop Natan Portela, BR-64002510 Teresina, Piaui - Brazil

^[4] Drugs Neglected Dis Initiat DNDi, BR-20010020 Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 9, n. 6 JUN 2021.
• Citações Web of Science: 0

Resumo

Treatment of visceral leishmaniasis in Brazil still relies on meglumine antimoniate, with less than ideal efficacy and safety, making new therapeutic tools an urgent need. The oral drug miltefosine was assayed in a phase II clinical trial in Brazil with cure rates lower than previously demonstrated in India. The present study investigated the susceptibility to miltefosine in 73 Brazilian strains of Leishmania infantum from different geographic regions, using intracellular amastigote and promastigote assays. The EC50 for miltefosine of 13 of these strains evaluated in intracellular amastigotes varied between 1.41 and 4.57 mu M. The EC50 of the 73 strains determined in promastigotes varied between 5.89 and 23.7 mu M. No correlation between in vitro miltefosine susceptibility and the presence of the miltefosine sensitive locus was detected among the tested strains. The relatively low heterogeneity in miltefosine susceptibility observed for the 73 strains tested in this study suggests the absence of decreased susceptibility to miltefosine in Brazilian L. infantum and does not exclude future clinical evaluation of miltefosine for VL treatment in Brazil. (AU)

• Processo FAPESP: 16/23405-4 - Suscetibilidade à miltefosina em isolados clínicos de Leishmania (Viannia) braziliensis: caracterização fenotípica e investigação das bases envolvidas na redução de suscetibilidade ao fármaco
• Beneficiário: Caroline Ricce Espada
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto