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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Vitro Susceptibility to Miltefosine of Leishmania infantum (syn. L. chagasi) Isolates from Different Geographical Areas in Brazil

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Author(s):
Espada, Caroline Ricce [1] ; Levatti, Erica V. de Castro [1] ; Boite, Mariana Cortes [2] ; Lamounier, Dorcas [3] ; Alvar, Jorge [4] ; Cupolillo, Elisa [2] ; Costa, Carlos Henrique Nery [3] ; Rode, Joelle [4] ; Uliana, Silvia R. B. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508000 Sao Paulo - Brazil
[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Pesquisa Leishmanioses, BR-21040900 Rio De Janeiro - Brazil
[3] Univ Fed Piaui, Hosp Doencas Trop Natan Portela, BR-64002510 Teresina, Piaui - Brazil
[4] Drugs Neglected Dis Initiat DNDi, BR-20010020 Rio De Janeiro - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MICROORGANISMS; v. 9, n. 6 JUN 2021.
Web of Science Citations: 0
Abstract

Treatment of visceral leishmaniasis in Brazil still relies on meglumine antimoniate, with less than ideal efficacy and safety, making new therapeutic tools an urgent need. The oral drug miltefosine was assayed in a phase II clinical trial in Brazil with cure rates lower than previously demonstrated in India. The present study investigated the susceptibility to miltefosine in 73 Brazilian strains of Leishmania infantum from different geographic regions, using intracellular amastigote and promastigote assays. The EC50 for miltefosine of 13 of these strains evaluated in intracellular amastigotes varied between 1.41 and 4.57 mu M. The EC50 of the 73 strains determined in promastigotes varied between 5.89 and 23.7 mu M. No correlation between in vitro miltefosine susceptibility and the presence of the miltefosine sensitive locus was detected among the tested strains. The relatively low heterogeneity in miltefosine susceptibility observed for the 73 strains tested in this study suggests the absence of decreased susceptibility to miltefosine in Brazilian L. infantum and does not exclude future clinical evaluation of miltefosine for VL treatment in Brazil. (AU)

FAPESP's process: 16/23405-4 - Susceptibility to miltefosine in Leishmania (Viannia) braziliensis clinical isolates: phenotypic characterization and investigation of the basis involved in reduced susceptibility to the drug
Grantee:Caroline Ricce Espada
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)