Effects of Baccharin Isolated from Brazilian Green Propolis on Adipocyte Differentiation and Hyperglycemia in ob/ob Diabetic Mice

Autor(es)

Watanabe, Akio ^{[1, 2]} ; de Almeida, Marilia Oliveira ^[3] ; Deguchi, Yusuke ^[4] ; Kozuka, Ryuzo ^[4] ; Arruda, Caroline ^[3] ; Berretta, Andresa Aparecida ^[5] ; Bastos, Jairo Kenupp ^[3] ; Woo, Je-Tae ^{[1, 4]} ; Yonezawa, Takayuki ^[1]

Afiliação do(s) autor(es)

^[1] Chubu Univ, Res Inst Biol Funct, Kasugai, Aichi 4878501 - Japan

^[2] Jumonji Univ, Fac Human Life, Dept Food Sci, Saitama 3528510 - Japan

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Chubu Univ, Dept Biol Chem, Kasugai, Aichi 4878501 - Japan

^[5] APIS FLORA INDL COML LTDA, Lab Res Dev & Innovat, BR-14020670 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 13 JUL 2021.
• Citações Web of Science: 0

Resumo

Propolis is a honeybee product with various biological activities, including antidiabetic effects. We previously reported that artepillin C, a prenylated cinnamic acid derivative isolated from Brazilian green propolis, acts as a peroxisome proliferator-activated receptor gamma (PPAR gamma) ligand and promotes adipocyte differentiation. In this study, we examined the effect of baccharin, another major component of Brazilian green propolis, on adipocyte differentiation. The treatment of mouse 3T3-L1 preadipocytes with baccharin resulted in increased lipid accumulation, cellular triglyceride levels, glycerol-3-phosphate dehydrogenase activity, and glucose uptake. The mRNA expression levels of PPAR gamma and its target genes were also increased by baccharin treatment. Furthermore, baccharin enhanced PPAR gamma-dependent luciferase activity, suggesting that baccharin promotes adipocyte differentiation via PPAR gamma activation. In diabetic ob/ob mice, intraperitoneal administration of 50 mg/kg baccharin significantly improved blood glucose levels. Our results suggest that baccharin has a hypoglycemic effect on glucose metabolic disorders, such as type 2 diabetes mellitus. (AU)

• Processo FAPESP: 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático