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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Virome comparison of deferred blood donations obtained from different geographic regions in the Sao Paulo State, Brazil

Texto completo
Autor(es):
Bezerra, Rafael dos Santos [1] ; Bub, Carolina Bonet [2] ; Peronni, Kamila Chagas [3] ; de Figiueiredo Barros, Bruna Duraes [4] ; da Costa Lira, Sanny Marcele [5] ; Kutner, Jose Mauro [2] ; Covas, Dimas Tadeu [1] ; Kashima, Simone [1] ; Slavov, Svetoslav Nanev [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Blood Ctr Ribeirao Preto, 2501 Tentente Catao Roxo St, BR-14051140 Ribeirao Preto, SP - Brazil
[2] Hosp Albert Einstein, Sao Paulo, SP - Brazil
[3] IPEC, Inst Canc Res, Guarapuava, Parana - Brazil
[4] Int Res Ctr AC Camargo, Genome Sequencing Facil, Sao Paulo, SP - Brazil
[5] Inst Hlth Strateg Management Fed Dist IGESF, Brasilia, DF - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: TRANSFUSION AND APHERESIS SCIENCE; v. 60, n. 3 JUN 2021.
Citações Web of Science: 0
Resumo

The virome composition of blood units deferred due to symptomatic disease of the donors reported after blood donation may reveal novel or unsuspected viral agents which may have impact in the area of hemotherapy. The objective of this study was to compare the virome of blood donations obtained from two distantly located blood collecting institutions in the Saqo Paulo State and deferred from use due to post donation illness reports (PDIR). Plasma samples with PDIR due to different symptoms were collected in two cities of the Sao Paulo State (Sao Paulo city, 28 samples and Ribeirao Preto city, 11 samples). The samples were assembled in pools and sequenced in Illumina NextSeq 550 sequencer. The obtained raw sequencing data was analyzed using bioinformatic pipeline aiming viral identification. Phylogenetic classification of the most important contigs was also performed. The virome composition of the plasma samples obtained in both cities was different. This was more pronounced for some specific anellovirus types and the human pegivirus-1 (HPgV-1) which were exclusively found among donations obtained from the city of Sao Paulo. On the other hand, in PDIR samples from Ribeirao Preto, Dengue -2 reads were more abundant compared to commensal viral representatives. The obtained virome findings show that the differential viral abundance is related to geographic localization and specific disease endemicity. The virome of PDIR samples may be used to more profoundly analyze the hypothetic transfusion threats in a given location. (AU)

Processo FAPESP: 17/23205-8 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas
Beneficiário:Svetoslav Nanev Slavov
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 19/08528-0 - Aplicação de métodos de bioinformática para identificação de infecções virais com impacto em hemoterapia
Beneficiário:Rafael dos Santos Bezerra
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 19/07861-8 - Desenvolvimento de um pipeline de bioinformática para identificação de doenças infecciosas emergentes em pacientes em regime de transfusão crônica
Beneficiário:Ian Nunes Valença
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 18/15826-5 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas
Beneficiário:Svetoslav Nanev Slavov
Linha de fomento: Bolsas no Brasil - Apoio a Jovens Pesquisadores