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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Virome comparison of deferred blood donations obtained from different geographic regions in the Sao Paulo State, Brazil

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Author(s):
Bezerra, Rafael dos Santos [1] ; Bub, Carolina Bonet [2] ; Peronni, Kamila Chagas [3] ; de Figiueiredo Barros, Bruna Duraes [4] ; da Costa Lira, Sanny Marcele [5] ; Kutner, Jose Mauro [2] ; Covas, Dimas Tadeu [1] ; Kashima, Simone [1] ; Slavov, Svetoslav Nanev [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Blood Ctr Ribeirao Preto, 2501 Tentente Catao Roxo St, BR-14051140 Ribeirao Preto, SP - Brazil
[2] Hosp Albert Einstein, Sao Paulo, SP - Brazil
[3] IPEC, Inst Canc Res, Guarapuava, Parana - Brazil
[4] Int Res Ctr AC Camargo, Genome Sequencing Facil, Sao Paulo, SP - Brazil
[5] Inst Hlth Strateg Management Fed Dist IGESF, Brasilia, DF - Brazil
Total Affiliations: 5
Document type: Journal article
Source: TRANSFUSION AND APHERESIS SCIENCE; v. 60, n. 3 JUN 2021.
Web of Science Citations: 0
Abstract

The virome composition of blood units deferred due to symptomatic disease of the donors reported after blood donation may reveal novel or unsuspected viral agents which may have impact in the area of hemotherapy. The objective of this study was to compare the virome of blood donations obtained from two distantly located blood collecting institutions in the Saqo Paulo State and deferred from use due to post donation illness reports (PDIR). Plasma samples with PDIR due to different symptoms were collected in two cities of the Sao Paulo State (Sao Paulo city, 28 samples and Ribeirao Preto city, 11 samples). The samples were assembled in pools and sequenced in Illumina NextSeq 550 sequencer. The obtained raw sequencing data was analyzed using bioinformatic pipeline aiming viral identification. Phylogenetic classification of the most important contigs was also performed. The virome composition of the plasma samples obtained in both cities was different. This was more pronounced for some specific anellovirus types and the human pegivirus-1 (HPgV-1) which were exclusively found among donations obtained from the city of Sao Paulo. On the other hand, in PDIR samples from Ribeirao Preto, Dengue -2 reads were more abundant compared to commensal viral representatives. The obtained virome findings show that the differential viral abundance is related to geographic localization and specific disease endemicity. The virome of PDIR samples may be used to more profoundly analyze the hypothetic transfusion threats in a given location. (AU)

FAPESP's process: 17/23205-8 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 19/08528-0 - Application of bioinformatic procedures for identification of the impact of the viral infections in hemotherapy
Grantee:Rafael dos Santos Bezerra
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 19/07861-8 - Development of a bioinformatic pipeline for identification of emerging infectious diseases among patients in regimen of chronic transfusion
Grantee:Ian Nunes Valença
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/15826-5 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem-cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support Opportunities: Scholarships in Brazil - Young Researchers