Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach

• Grant number: 17/23205-8
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: August 01, 2018
• End date: July 31, 2024
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Svetoslav Nanev Slavov
• Grantee: Svetoslav Nanev Slavov
• Host Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil
• Associated researchers: Ana Cristina Silva Pinto ; Anna Maria Eis-Huebinger ; Belinda Pinto Simoes ; Caro Valerie ; Clarisse Martins Machado ; Dimas Tadeu Covas ; Gil Cunha de Santis ; Jose Mauro Kutner ; Mathias Vandenbogaert ; Nelson Hamerschlak ; Sanny Marcele da Costa Lira ; Sauvage ; Simone Kashima Haddad ; Syria Laperche
• Associated scholarship(s): 23/12155-0 - Machine Learning Algorithms applied for Virus Identification in Dark Matter sequencing data from Next Generation Sequencing, BP.MS; 22/14958-0 - Molecular diagnosis and next-generation sequencing for emerging viral infections in different clinical samples, BP.TT; 22/10278-5 - Viral metagenomics in pediatric oncology patients presenting febrile neutropenia without etiologic diagnosis, BP.MS; + associated scholarships 21/09446-8 - Application of portable minion sequencing technology for viral metagenomics analysis, BP.MS; 22/00910-6 - Development and application of bioinformatic pipelines for metagenomic analysis of clinical samples, BP.IC; 21/10595-8 - Molecular evaluation of viruses of clinical interest in plasma samples of patients with stem-cell transplantation, BP.IC; 20/13194-1 - Evaluation of the prevalence and application of next generation sequencing methods for identification of emerging viruses in the area of hemotherapy, BP.TT; 19/07861-8 - Development of a bioinformatic pipeline for identification of emerging infectious diseases among patients in regimen of chronic transfusion, BP.MS; 19/08528-0 - Application of bioinformatic procedures for identification of the impact of the viral infections in hemotherapy, BP.MS; 18/22009-3 - Activities of technical support in the collection of samples and molecular techniques in patients with sickle cell disease submitted to multiple transfusions, BP.TT; 18/15826-5 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem-cell transplantation by multiple-research approach, BP.JP - associated scholarships

Abstract

In the last decades, the scientific attention has been largely turned to a specific area of the clinical virology comprised by the emerging and reemerging viruses. This is due to the fact that these infectious agents have been recognized as a continuing threat to human health. Many of them can be transmitted by hemotherapy procedures including application of hemoderivatives or hematopoietic stem cell transplantations (HSCT). In the majority of cases, the emerging infections are represented by zoonotic viruses with exotic natural cycles, which are transmitted by insect vectors. Their dissemination in the human population can be related to high morbidity outbreaks with many asymptomatic infections which in turn can impact the hemotherapy process. In Brazil, there have been no comprehensive studies of such infections and their real threat for the hemotherapy processes is underestimated and largely unknown. This proposal, therefore, aims to investigate by multiple-based approaches including viral metagenomics next generation sequencing and state-of-the art molecular methods the significance of the emerging infections in the field of hemotherapy. For this purpose, the studies will be performed on plasma samples obtained from patients submitted to HSCT, multiply-transfused patients and deferred blood units. The project will also evaluate in a constant manner the prevalence of different viral infections in the blood donor population of our region which is subject to frequent arbovirus outbreaks. Therefore, the systematic approach of this project will add positive value for the understanding of the impact of the emerging viruses for the hemotherapy in Brazil and will capacitate Brazilian researches in the area of metagenomic analysis and next generation sequencing. This proposal represents a new research line in an already established scientific institution (Blood Center of Ribeirão Preto) and counts with national collaborations from important clinical institutions (Institute of Tropical Medicine, Hospital "Albert Einstein"-São Paulo, Faculty of Medicine of Ribeirão Preto-USP). Moreover, the project will be supported by many international collaborators from leading research clinical and bioinformatics centers from France and Germany (Pasteur Institute, National Institute of Blood Transfusion, Paris, France; Institute of Virology, University of Bonn, Germany), and aims at establishing a closely related research group for emerging viruses and hemotherapy/stem cell transplantation. (AU)

Scientific publications

(24)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BEZERRA, RAFAEL DOS SANTOS; BUB, CAROLINA BONET; PERONNI, KAMILA CHAGAS; DE FIGIUEIREDO BARROS, BRUNA DURAES; DA COSTA LIRA, SANNY MARCELE; KUTNER, JOSE MAURO; COVAS, DIMAS TADEU; KASHIMA, SIMONE; SLAVOV, SVETOSLAV NANEV. Virome comparison of deferred blood donations obtained from different geographic regions in the Sao Paulo State, Brazil. TRANSFUSION AND APHERESIS SCIENCE, v. 60, n. 3, JUN 2021. (17/23205-8, 19/08528-0, 19/07861-8, 18/15826-5)

RAFAEL DOS SANTOS BEZERRA; KAMILA CHAGAS PERONNI; BRUNA DURÃES DE FIGUEIREDO BARROS; LEONARDO SCALON DE OLIVEIRA; EVANDRA STRAZZA RODRIGUES; ROCHELE AZEVEDO; EUGÊNIA MARIA AMORIM UBIALI; DIMAS TADEU COVAS; SIMONE KASHIMA; SVETOSLAV NANEV SLAVOV. Viral metagenomics in blood donors with post-donation diseases and negative tests for dengue and Zika viruses RNA detection during a major outbreak of arboviruses in Sao Paulo State in 2016. Revista do Instituto de Medicina Tropical de São Paulo, v. 62, p. -, 2020. (19/08528-0, 18/15826-5, 19/07861-8, 17/23205-8)

BEZERRA, RAFAEL DOS SANTOS; XIMENEZ, JOAO PAULO BIANCHI; GIOVANETTI, MARTA; ZUCHERATO, VICTORIA SIMIONATTO; BITENCOURT, HELEN TAYANA; ZIMMERMANN, ANA; ALCANTARA JUNIOR, LUIZ CARLOS JUNIOR; COVAS, DIMAS TADEU; KASHIMA, SIMONE; SLAVOV, SVETOSLAV NANEV. Metavirome composition of Brazilian blood donors positive for the routinely tested blood-borne infections. VIRUS RESEARCH, v. 311, p. 7-pg., 2022-04-02. (20/13194-1, 17/23205-8, 19/08528-0, 18/15826-5)

SLAVOV, S. N.; MARANINCHI SILVEIRA, R.; HESPANHOL, M. R.; SAUVAGE, V.; RODRIGUES, E. S.; FONTANARI KRAUSE, L.; BITTENCOURT, H. T.; CARO, V.; LAPERCHE, S.; COVAS, D. T.; et al. Human pegivirus-1 (HPgV-1) RNA prevalence and genotypes in volunteer blood donors from the Brazilian Amazon. TRANSFUSION CLINIQUE ET BIOLOGIQUE, v. 26, n. 4, p. 234-239, NOV 2019. (09/16623-1, 17/23205-8, 14/50947-7)

TOSTA, STEPHANE; MORENO, KELDENN; SCHUAB, GABRIEL; FONSECA, VAGNER; SEGOVIA, FATIMA MARIA CARDOZO; KASHIMA, SIMONE; ELIAS, MARIA CAROLINA; SAMPAIO, SANDRA COCCUZZO; CICCOZZI, MASSIMO; JUNIOR ALCANTARA, LUIZ CARLOS; et al. Global SARS-CoV-2 genomic surveillance: What we have learned (so far). INFECTION GENETICS AND EVOLUTION, v. 108, p. 8-pg., 2023-03-01. (17/23205-8)

BEZERRA, RAFAEL S.; BITENCOURT, HELLEN T.; COVAS, DIMAS T.; KASHIMA, SIMONE; SLAVOV, SVETOSLAV N.. Metagenomic identification of human Gemykibivirus-2 (HuGkV-2) in parenterally infected blood donors from the Brazilian Amazon. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 98, p. 3-pg., 2020-09-01. (19/08528-0, 18/15826-5, 19/07861-8, 17/23205-8)

ZANETTE, DALILA LUCIOLA; COELHO, KAROLINE BRITO CAETANO ANDRADE; DE CARVALHO, ENEAS; AOKI, MATEUS NOBREGA; NARDIN, JEANINE MARIE; LALLI, LARISSA ARAUJO; BEZERRA, RAFAEL DOS SANTOS; GIOVANETTI, MARTA; ZUCHERATO, VICTORIA SIMIONATTO; DE CAMPOS, GABRIEL MONTENEGRO; et al. Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer. MOLECULAR & CELLULAR ONCOLOGY, v. 10, n. 1, p. 9-pg., 2023-12-31. (19/08528-0, 22/00910-6, 17/23205-8, 20/13194-1)

BEZERRA, RAFAEL DOS SANTOS; BITENCOURT, HELLEN TAYANA; COVAS, DIMAS TADEU; KASHIMA, SIMONE; SLAVOV, SVETOSLAV NANEV. Molecular evolution pattern of Merkel cell polyomavirus identified by viral metagenomics in plasma of high-risk blood donors from the Brazilian Amazon. INFECTION GENETICS AND EVOLUTION, v. 85, NOV 2020. (19/08528-0, 17/23205-8, 18/15826-5, 19/07861-8)

ARTHUR, RODRIGO ALEX; BEZERRA, RAFAEL DOS SANTOS; BIANCHI XIMENEZ, JOAO PAULO; MERLIN, BRUNA LAIS; MORRAYE, RAPHAEL DE ANDRADE; NETO, JOAO VALENTINI; NASSER FAVA, NATALIA MELO; ALVES FIGUEIREDO, DAVID LIVINGSTONE; OLIVEIRA DE BIAGI JR, CARLOS ALBERTO; MONTIBELLER, MARIA JARA; et al. Microbiome and oral squamous cell carcinoma: a possible interplay on iron metabolism and its impact on tumor microenvironment. Brazilian Journal of Microbiology, v. 52, n. 3, p. 1287-1302, SEP 2021. (19/08528-0, 17/23205-8, 13/08135-2)

VALENCA, IAN NUNES; BEZERRA, RAFAEL DOS SANTOS; DE OLIVEIRA, LUCIANA CORREA OLIVEIRA; COVAS, DIMAS TADEU; KASHIMA, SIMONE; SLAVOV, SVETOSLAV NANEV. Deep viral metagenomics in patients with haemophilia receiving plasma-derived coagulation factor concentrates. HAEMOPHILIA, v. 27, n. 5, JUL 2021. (20/13194-1, 17/23205-8, 19/08528-0, 19/07861-8)

SLAVOV, SVETOSLAV N.; MACONETTO, JULIANA D. M.; MARTINEZ, EDSON Z.; SILVA-PINTO, ANA CRISTINA; COVAS, DIMAS T.; EIS-HUEBINGER, ANNA MARIA; KASHIMA, SIMONE. Prevalence of hepatitis E virus infection in multiple transfused Brazilian patients with thalassemia and sickle cell disease. Journal of Medical Virology, v. 91, n. 9, p. 1693-1697, SEP 2019. (09/16623-1, 17/23205-8, 14/50947-7)

SLAVOV, SVETOSLAV NANEV; SILVEIRA, ROBERTA MARANINCHI; RODRIGUES, EVANDRA STRAZZA; DIEFENBACH, CRISTIANE FRACAO; ZIMMERMANN, ANA MARIA; COVAS, DIMAS TADEU; KASHIMA, SIMONE. Human pegivirus-1 pegivirus-1 (HPgV-1, GBV-C) RNA prevalence and genotype diversity among volunteer blood donors from an intra-hospital hemotherapy service in Southern Brazil. TRANSFUSION AND APHERESIS SCIENCE, v. 58, n. 2, p. 174-178, APR 2019. (14/50947-7, 17/23205-8, 09/16623-1)

SLAVOV, SVETOSLAV NANEV; GUARAGNA MACHADO, RAFAEL RAHAL; FERREIRA, ALEXANDER RODRIGO; SOARES, CAMILA PEREIRA; ARAUJO, DANIELLE BASTOS; LEAL OLIVEIRA, DANIELLE BRUNA; COVAS, DIMAS TADEU; DURIGON, EDISON LUIZ; KASHIMA, SIMONE. Zika virus seroprevalence in blood donors from the Northeastern region of Sao Paulo State, Brazil, between 2015 and 2017. Journal of Infection, v. 80, n. 1, p. 111-115, JAN 2020. (17/23205-8, 16/20045-7, 16/15049-3, 16/08727-5, 14/50947-7)

SLAVOV, SVETOSLAV N.; RODRIGUES, EVANDRA S.; SAUVAGE, VIRGINIE; CARO, VALERIE; DIEFENBACH, CRISTIANE F.; ZIMMERMANN, ANA M.; COVAS, DIMAS T.; LAPERCHE, SYRIA; KASHIMA, SIMONE. Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil. Journal of Medical Virology, v. 91, n. 7, p. 1224-1231, JUL 2019. (09/16623-1, 17/23205-8, 14/50947-7)

ZUCHERATO, VICTORIA SIMIONATTO; DA COSTA, PERICLES NATAN MENDES; GIOVANETTI, MARTA; KRAUSE, LUCIANA MARIA FONTANARI; ALVES, DAIANI CRISTINA CILIAO; MOREIRA, RENATA MARIA ALENCAR; PIMENTEL, BARBARA MACIEL SIDOU; HADDAD, RODRIGO; BITENCOURT, HELLEN TAYANA; CICCOZZI, MASSIMO; et al. Merkel cell polyomavirus (MCPyV) DNA prevalence in Brazilian blood donors. TRANSFUSION CLINIQUE ET BIOLOGIQUE, v. 30, n. 1, p. 4-pg., 2023-01-31. (20/13194-1, 17/23205-8, 18/15826-5)

BEZERRA, RAFAEL DOS SANTOS; DE MELO JORGE, DANIEL MACEDO; CASTRO, ITALO ARAUJO; MORETTO, EDSON LARA; DE OLIVEIRA, LEONARDO SCALON; AMORIM UBIALI, EUGENIA MARIA; COVAS, DIMAS TADEU; ARRUDA, EURICO; KASHIMA, SIMONE; SLAVOV, SVETOSLAV NANEV. Detection of Influenza A(H3N2) Virus RNA in Donated Blood. Emerging Infectious Diseases, v. 26, n. 7, p. 1621-1623, JUL 2020. (17/23205-8, 19/07861-8, 18/15826-5, 19/08528-0)

ZUCHERATO, VICTORIA SIMIONATTO; EVARISTO, MARIANE; SANTOS, ELAINE VIEIRA; MELLO, RALYRIA; CANDIDO, ERIKA DONIZETTI; ARAUJO, DANIELLE BASTOS; GIOVANETTI, MARTA; ALCANTARA, LUIZ CARLOS JUNIOR; CILIAO-ALVES, DAIANI CRISTINA; HADDAD, RODRIGO; et al. SARS-CoV-2 serological cross-reactivity testing in Brazilian blood donors, October-December, 2019. Journal of Infection, v. 84, n. 2, p. 2-pg., 2022-02-01. (20/13194-1, 17/23205-8)

ALVES FIGUEIREDO, DAVID LIVINGSTONE; BIANCHI XIMENEZ, JOAO PAULO; FERREIRA SEIVA, FABIO RODRIGUES; PANIS, CAROLINA; BEZERRA, RAFAEL DOS SANTOS; FERRASA, ADRIANO; CECCHINI, ALESSANDRA LOURENCO; DE MEDEIROS, ALEXANDRA IVO; ALMEIDA, ANA MARISA FUSCO; RAMAO, ANELISA; et al. COVID-19: The question of genetic diversity and therapeutic intervention approaches. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 1, p. 19-pg., 2021-01-01. (17/23205-8, 19/08528-0, 13/08135-2)

I.N. VALENÇA; F.A. RÓS; V.S. ZUCHERATO; A.C. SILVA-PINTO; D.T. COVAS; S. KASHIMA; S.N. SLAVOV. Comparative metavirome analysis in polytransfused patients. Brazilian Journal of Medical and Biological Research, v. 54, n. 12, 2021-10-18. (20/13194-1, 19/22155-2, 18/15826-5, 17/23205-8, 19/07861-8)

BEZERRA, RAFAEL DOS SANTOS; DIEFENBACH, CRISTIANE FRACAO; PEREIRA, DALNEI VEIGA; KASHIMA, SIMONE; SLAVOV, SVETOSLAV NANEV. Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil. Brazilian Journal of Infectious Diseases, v. 24, n. 3, p. 250-255, MAY-JUN 2020. (19/07861-8, 18/15826-5, 19/08528-0, 17/23205-8)

VALENCA, IAN NUNES; DOS SANTOS, RAFAEL BEZERRA; PERONNI, KAMILA CHAGAS; SAUVAGE, VIRGINIE; VANDENBOGAERT, MATHIAS; CARO, VALERIE; DA SILVA JUNIOR, WILSON ARAUJO; COVAS, DIMAS TADEU; SILVA-PINTO, ANA CRISTINA; LAPERCHE, SYRIA; et al. Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia. Revista do Instituto de Medicina Tropical de São Paulo, v. 63, 2021. (19/07861-8, 18/15826-5, 19/08528-0, 17/23205-8)

NUNES VALENCA, I; SILVA-PINTO, A. C.; ARAUJO DA SILVA JUNIOR, W.; COVAS, D. TADEU; KASHIMA, S.; NANEV SLAVOV, S.. Viral metagenomics in Brazilian multiply transfused patients with sickle cell disease as an indicator for blood transfusion safety. TRANSFUSION CLINIQUE ET BIOLOGIQUE, v. 27, n. 4, p. 237-242, NOV 2020. (18/22009-3, 17/23205-8, 19/07861-8, 18/15826-5, 19/08528-0)

SLAVOV, S. N.; CHRISTOVA, I. S.; FERREIRA, A. R.; RODRIGUES, E. S.; BIANQUINI, M. L.; HESPANHOL, M. R.; COVAS, D. T.; KASHIMA, S.. Serological evidence of Borrelia circulation among blood donors in the Sao Paulo state, Brazil. Transfusion Medicine, v. 29, n. 5, p. 358-363, OCT 2019. (17/23205-8, 14/50947-7)

CAVALCANTE SOUZA, JULIANA VANESSA; SANTOS, HAZERRAL DE OLIVEIRA; LEITE, ANDERSON BRANDAO; GIOVANETTI, MARTA; BEZERRA, RAFAEL DOS SANTOS; DE CARVALHO, ENEAS; TODAO BERNARDINO, JARDELINA DE SOUZA; VIALA, VINCENT LOUIS; HADDAD, RODRIGO; CICCOZZI, MASSIMO; et al. Viral Metagenomics for the Identification of Emerging Infections in Clinical Samples with Inconclusive Dengue, Zika, and Chikungunya Viral Amplification. Viruses-Basel, v. 14, n. 9, p. 8-pg., 2022-09-01. (17/23205-8, 18/15826-5, 21/09446-8, 21/11944-6, 20/13194-1, 19/08528-0)