Dextran Sulfate/Pramlintide Polyelectrolyte Nanoparticles as a Promising Delivery System: Optimization, Evaluation of Supramolecular Interactions and Effect on Conformational Stability of the Peptide Drug

Autor(es)

Zuglianello, Carine ^[1] ; Chamorro, Andres F. ^[2] ; de Oliveira, Vanessa A. ^[3] ; Xavier Junior, Francisco H. ^[4] ; Lemos-Senna, Elenara ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Catarina, Dept Ciencias Farmaceut, Programa Posgrad Nanotecnol Farmaceut, Campus Trindade, BR-88040900 Florianopolis, SC - Brazil

^[2] Univ Fed Santa Catarina, Dept Quim, Programa Posgrad Quim, Campus Trindade, BR-88040900 Florianopolis, SC - Brazil

^[3] Univ Fed Santa Catarina, Lab Cent Biol Mol & Estrutural, Campus Trindade, BR-88040900 Florianopolis, SC - Brazil

^[4] Univ Fed Paraiba, Lab Biotecnol Farmaceut, Dept Ciencias Farmaceut, BR-58051900 Joao Pessoa, PB - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 32, n. 8, p. 1642-1653, AUG 2021.
• Citações Web of Science: 0

Resumo

In this study, we investigated the feasibility to obtain nanoparticles (NPs) by assembling pramlintide (Pram) with dextran sulfate (DexS), as a new approach for mucosal peptide delivery. DexS/Pram NPs were prepared by dropwise addition of a Pram solution to a DexS solution under magnetic stirring. The physicochemical characteristics of NPs and molecular interactions involved in the co-assembling were evaluated by dynamic light scattering (DLS), transmission electronic microscopy (TEM), isothermal titration microcalorimetry, Fourier-transform infrared spectroscopy (FTIR), fluorescence quenching, and circular dichroism (CD). DexS/Pram NPs displayed a narrow size distribution (ca. 200 nm), negative zeta potential (ca. −40 mV), association efficiency close to 100%, and nanogel behavior. The assembling with DexS increased the Pram α-helical content, stabilizing the peptide in its bioactive form. The colloidal stability of nanoparticles was dependent on the salt concentration and it could be assumed that peptide release from nanoparticles occurs by dissociation of the complex at physiological conditions. (AU)

• Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
• Beneficiário: Maria Vitória Lopes Badra Bentley
• Modalidade de apoio: Auxílio à Pesquisa - Temático