Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses

Autor(es):Mostrar menos -

Lourenco, Juliana D. ^[1] ; Teodoro, Walcy R. ^[2] ; Barbeiro, Denise F. ^[3] ; Velosa, Ana Paula P. ^[2] ; Silva, Larissa E. F. ^[1] ; Kohler, Julia B. ^[1] ; Moreira, Alyne R. ^[1] ; Aun, V, Marcelo ; da Silva, Isadora C. ^[4] ; Fernandes, Frederico L. A. ^[4] ; Negri, Elnara M. ^[5] ; Gross, Jefferson L. ^[6] ; Tiberio, Iolanda F. L. C. ^[1] ; Ito, Juliana T. ^[1] ; Lopes, Fernanda D. T. Q. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Clin Med, Lab Expt Therapeut, Av Dr Arnaldo 455 Room 1220, BR-01246903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin FMUSP, Fac Med, Rheumatol Div, Av Dr Arnaldo 455 Room 3124, BR-01246903 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Clin Med, Lab Clin Emergencies, Av Dr Arnaldo 455 Room 3132, BR-01246903 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Heart Inst, Pulm Div, Hosp Clin, Sch Med, Av Dr Eneas Carvalho Aguiar 44, BR-05403000 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Pathol, Lab Cellular Biol, Av Dr Arnaldo 455 Room 4349, BR-01246903 Sao Paulo - Brazil

^[6] AC Camargo Canc Ctr, Dept Thorac Surg, R Prof Antonio Prudente 211, BR-01509010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 10, n. 7 JUL 2021.
• Citações Web of Science: 0

Resumo

Th17/Treg imbalance plays a pivotal role in COPD development and progression. We aimed to assess Th17/Treg-related intracellular signaling at different COPD stages in local and systemic responses. Lung tissue and/or peripheral blood samples were collected and divided into non-obstructed (NOS), COPD stages I and II, and COPD stages III and IV groups. Gene expression of STAT3 and -5, ROR gamma t, Foxp3, interleukin (IL)-6, -17, -10, and TGF-beta was assessed by RT-qPCR. IL-6, -17, -10, and TGF-beta levels were determined by ELISA. We observed increased STAT3, ROR gamma t, Foxp3, IL-6, and TGF-beta gene expression and IL-6 levels in the lungs of COPD I and II patients compared to those of NOS patients. Regarding the systemic response, we observed increased STAT3, ROR gamma t, IL-6, and TGF-beta gene expression in the COPD III and IV group and increased IL-6 levels in the COPD I and II group. STAT5 was increased in COPD III and IV patients, although there was a decrease in Foxp3 expression and IL-10 levels in the COPD I and II and COPD III and IV groups, respectively. We demonstrated that an increase in Th17 intracellular signaling in the lungs precedes this increase in the systemic response, whereas Treg intracellular signaling varies between the compartments analyzed in different COPD stages. (AU)

• Processo FAPESP: 16/17817-8 - O papel das proteínas SOCS e STAT na diferenciação das células TCD4+ naive no desenvolvimento da doença pulmonar obstrutiva crônica (DPOC) em pacientes fumantes e em modelo experimental de exposição à fumaça de cigarro
• Beneficiário: Fernanda Degobbi Tenorio Quirino dos Santos Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/21408-6 - O papel das proteínas socs e stats na diferenciação das células tcd4+ naive no desenvolvimento da doença pulmonar obstrutiva crônica: comparação entre a resposta imunológica local e sistêmica
• Beneficiário: Juliana Dias Lourenço
• Modalidade de apoio: Bolsas no Brasil - Doutorado