Antenna regeneration as an ecotoxicological endpoint in a marine amphipod: a proof of concept using dimethyl sulfoxide and diflubenzuron

Autor(es)

Diehl, Otavio J. ^[1] ; Assano, Patricia K. ^[1] ; da Costa, Thiago Roncini G. ^[2] ; Oliveira, Rhaul ^[1] ; Marques-Souza, Henrique ^[2] ; Umbuzeiro, Gisela de A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Technol, BR-13484332 Limeira - Brazil

^[2] Univ Estadual Campinas, Inst Biol, BR-13083862 Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY; v. 30, n. 4, p. 751-755, MAY 2021.
• Citações Web of Science: 0

Resumo

Regeneration is a widely spread process across the animal kingdom, including many species of marine crustaceans. It is strongly linked to hormonal cycles and, therefore, a great endpoint candidate for toxicology studies. We selected the amphipod Parhyale hawaiensis as test organism, already used in ecotoxicological studies and able to regenerate its body appendages. We are proposing a protocol to use the antenna regeneration as a toxicity endpoint. First, we evaluated differences in time of completion of regeneration in males and females after the amputation of one antenna of 6 months old animals. Then we compared the influence of different testing volumes in the regeneration process (100 and 5 mL). We used as testing substances, dimethyl sulfoxide (DMSO) and diflubenzuron, a chitin synthesis inhibitor. The most suitable protocol consisted of volumes of 5 mL in 12-well microplates, with 1 organism per well, 12 organisms per concentration (1:1 females/males) and test time duration of around 5 weeks. DMSO accelerated regeneration time with a NOEC of 0.06%. Diflubenzuron inhibited the time necessary to its completion with a NOEC of 0.32 mu g L-1. We conclude that the Parhyale hawaiensis antenna regeneration protocol proposed here is a potential tool in ecotoxicology, but more studies are required for its validation not only to verify its utility for testing chemicals but also environmental samples. (AU)

• Processo FAPESP: 12/09602-0 - Caracterização da rede de regulação cardiogênica controlada por Coup-TFII a partir de células-tronco embrionárias
• Beneficiário: Henrique Marques Barbosa de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/23606-5 - Silenciamento gênico por RNAi do percevejo marrom Euschistus heros visando o controle de pragas na cultura da soja
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• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Beneficiário: Rhaul de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado