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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antenna regeneration as an ecotoxicological endpoint in a marine amphipod: a proof of concept using dimethyl sulfoxide and diflubenzuron

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Author(s):
Diehl, Otavio J. [1] ; Assano, Patricia K. [1] ; da Costa, Thiago Roncini G. [2] ; Oliveira, Rhaul [1] ; Marques-Souza, Henrique [2] ; Umbuzeiro, Gisela de A. [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Sch Technol, BR-13484332 Limeira - Brazil
[2] Univ Estadual Campinas, Inst Biol, BR-13083862 Campinas - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ECOTOXICOLOGY; v. 30, n. 4, p. 751-755, MAY 2021.
Web of Science Citations: 0
Abstract

Regeneration is a widely spread process across the animal kingdom, including many species of marine crustaceans. It is strongly linked to hormonal cycles and, therefore, a great endpoint candidate for toxicology studies. We selected the amphipod Parhyale hawaiensis as test organism, already used in ecotoxicological studies and able to regenerate its body appendages. We are proposing a protocol to use the antenna regeneration as a toxicity endpoint. First, we evaluated differences in time of completion of regeneration in males and females after the amputation of one antenna of 6 months old animals. Then we compared the influence of different testing volumes in the regeneration process (100 and 5 mL). We used as testing substances, dimethyl sulfoxide (DMSO) and diflubenzuron, a chitin synthesis inhibitor. The most suitable protocol consisted of volumes of 5 mL in 12-well microplates, with 1 organism per well, 12 organisms per concentration (1:1 females/males) and test time duration of around 5 weeks. DMSO accelerated regeneration time with a NOEC of 0.06%. Diflubenzuron inhibited the time necessary to its completion with a NOEC of 0.32 mu g L-1. We conclude that the Parhyale hawaiensis antenna regeneration protocol proposed here is a potential tool in ecotoxicology, but more studies are required for its validation not only to verify its utility for testing chemicals but also environmental samples. (AU)

FAPESP's process: 12/09602-0 - Characterization of the gene regulatory network controled by Coup-TFII in cardiomyocytes derived from embryonic stem cells
Grantee:Henrique Marques Barbosa de Souza
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/23606-5 - RNAi-mediate gene silencing of the brown stink bug Euschistus heros for pest control in soybean crop
Grantee:Henrique Marques Barbosa de Souza
Support Opportunities: Regular Research Grants
FAPESP's process: 18/03108-0 - Zebrafish embryo test as a tool for an integrated assessment of water quality in sugarcane impacted areas
Grantee:Rhaul de Oliveira
Support Opportunities: Scholarships in Brazil - Post-Doctoral