Anti-inflammatory Properties of Ethanolic Extract and 2 `'-O-beta-d-Glucopyranosyl-vitexin Obtained from Alternanthera tenella Colla Whole Plant

Autor(es)

Kassuya, Roberto Mikio ^[1] ; dos Santos, Elisangela ^[1] ; Bosso, Fernando Henrique ^[2] ; Pedroso, Taise Fonseca ^[2] ; Marinho, Jane Vasconcelos Neves ^[2] ; Salvador, Marcos Jose ^[2] ; Kassuya, Candida Aparecida Leite ^[1] ; Gasparotto Junior, Arquimedes ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Grande Dourados, Coll Hlth Sci, Dourados, MS - Brazil

^[2] Univ Campinas UNICAMP, PPG BTPB PPG BV & PPG BCE, Dept Plant Biol, Inst Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Inflammation; v. 44, n. 4, p. 1540-1552, AUG 2021.
• Citações Web of Science: 0

Resumo

Six flavonoids were identified and isolated from the ethanolic extract of Alternanthera tenella Colla (Amaranthaceae) whole plant (EEAT) including 2 `'-O-beta-d-glucopyranosyl-vitexin (A19). Flavonoids have anti-inflammatory activity; however, the 2 `'-O-beta-d-glucopyranosyl-vitexin anti-inflammatory property was not totally explored. The aim of the present study was to investigate the anti-inflammatory effects of ethanolic extract from A. tenella whole plant and isolated flavone C-glycoside A19 in models of inflammation. The oral administration (p.o.) of EEAT (30, 100, and 300 mg/kg), A19 (0.1, 1, and 10 mg/kg), and prednisolone (3 mg/kg) were tested against the carrageenan-induced paw edema in Swiss mice. The EEAT (100 mg/kg, p.o.), A19 (1 mg/kg, p.o.), and prednisolone (3 mg/kg, p.o.) were tested in the zymosan-articular inflammation, carrageenan-pleurisy, and complete Freund's adjuvant (CFA) models in Swiss mice. In silico analysis and search for structural relationships between vitexin derivatives flavones present in the EEAT and TNF-alpha inhibitors were performed. EEAT, A19, and prednisolone significantly inhibited (i) edema, mechanical hyperalgesia in carrageenan-induced paw inflammation; (ii) leukocyte migration and protein extravasation in carrageenan-induced pleurisy; (iii) knee edema, mechanical hyperalgesia, and leukocyte migration in articular inflammation induced by zymosan. Still the CFA induced the increase in myeloperoxidase and N-acetylglucosaminidase activities, EEAT, A19, and prednisolone significantly inhibited these enzymes. The in silico analysis showed that molecules with similar structure to apigenin and vitexin have a potential inhibition on the TNF system. This study confirms the anti-inflammatory properties of EEAT and A19. The C-glycosylated flavone A19 could be responsible for the EEAT anti-edematogenic and anti-hyperalgesic effects and a potential TNF-alpha inhibitor. (AU)

• Processo FAPESP: 17/09781-6 - Estudo comparativo do emprego de reator encamisado, ultrassom e maceração no preparo de extratos padronizados de Alternanthera paronychioides e avaliação da atividade antioxidante
• Beneficiário: Fernando Henrique Bosso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/03726-8 - Estudo químico e biológico integrado para a busca de substâncias bioativas em espécies vegetais* e desenvolvimento de procedimentos analíticos. *(Amaranthaceae, Gesneriaceae, Myrtaceae e Annonaceae)
• Beneficiário: Marcos José Salvador
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/06079-4 - Busca de antioxidantes e antimicrobianos em espécies da tribo Gomphreneae
• Beneficiário: Marcos José Salvador
• Modalidade de apoio: Auxílio à Pesquisa - Regular