| Full text | |
| Author(s): |
Kassuya, Roberto Mikio
[1]
;
dos Santos, Elisangela
[1]
;
Bosso, Fernando Henrique
[2]
;
Pedroso, Taise Fonseca
[2]
;
Marinho, Jane Vasconcelos Neves
[2]
;
Salvador, Marcos Jose
[2]
;
Kassuya, Candida Aparecida Leite
[1]
;
Gasparotto Junior, Arquimedes
[1]
Total Authors: 8
|
| Affiliation: | [1] Fed Univ Grande Dourados, Coll Hlth Sci, Dourados, MS - Brazil
[2] Univ Campinas UNICAMP, PPG BTPB PPG BV & PPG BCE, Dept Plant Biol, Inst Biol, Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Inflammation; v. 44, n. 4, p. 1540-1552, AUG 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Six flavonoids were identified and isolated from the ethanolic extract of Alternanthera tenella Colla (Amaranthaceae) whole plant (EEAT) including 2 `'-O-beta-d-glucopyranosyl-vitexin (A19). Flavonoids have anti-inflammatory activity; however, the 2 `'-O-beta-d-glucopyranosyl-vitexin anti-inflammatory property was not totally explored. The aim of the present study was to investigate the anti-inflammatory effects of ethanolic extract from A. tenella whole plant and isolated flavone C-glycoside A19 in models of inflammation. The oral administration (p.o.) of EEAT (30, 100, and 300 mg/kg), A19 (0.1, 1, and 10 mg/kg), and prednisolone (3 mg/kg) were tested against the carrageenan-induced paw edema in Swiss mice. The EEAT (100 mg/kg, p.o.), A19 (1 mg/kg, p.o.), and prednisolone (3 mg/kg, p.o.) were tested in the zymosan-articular inflammation, carrageenan-pleurisy, and complete Freund's adjuvant (CFA) models in Swiss mice. In silico analysis and search for structural relationships between vitexin derivatives flavones present in the EEAT and TNF-alpha inhibitors were performed. EEAT, A19, and prednisolone significantly inhibited (i) edema, mechanical hyperalgesia in carrageenan-induced paw inflammation; (ii) leukocyte migration and protein extravasation in carrageenan-induced pleurisy; (iii) knee edema, mechanical hyperalgesia, and leukocyte migration in articular inflammation induced by zymosan. Still the CFA induced the increase in myeloperoxidase and N-acetylglucosaminidase activities, EEAT, A19, and prednisolone significantly inhibited these enzymes. The in silico analysis showed that molecules with similar structure to apigenin and vitexin have a potential inhibition on the TNF system. This study confirms the anti-inflammatory properties of EEAT and A19. The C-glycosylated flavone A19 could be responsible for the EEAT anti-edematogenic and anti-hyperalgesic effects and a potential TNF-alpha inhibitor. (AU) | |
| FAPESP's process: | 17/09781-6 - Comparative study of the use of reactor, ultrasound and maceration in the preparation of standardized extracts of Alternanthera paronychioides and evaluation of antioxidant activity |
| Grantee: | Fernando Henrique Bosso |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 15/03726-8 - Integrated chemical and biological study in the search for bioactive substances in plants* and development of analytical procedures. *(Amaranthaceae, Gesneriaceae, Myrtaceae e Annonaceae) |
| Grantee: | Marcos José Salvador |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 06/06079-4 - Search of antioxidants and antimicrobials in Gomphreneae tribe species |
| Grantee: | Marcos José Salvador |
| Support Opportunities: | Regular Research Grants |