MicroRNA-mediated extracellular matrix remodeling in squamous cell carcinoma of the oral cavity

Autor(es):Mostrar menos -

Menderico Junior, Gilberto Mendes ^[1] ; Theodoro, Therese Rachell ^[2] ; Pasini, Fatima Solange ^[3] ; de Menezes Ishikawa, Marina ^[4] ; Santos, Nayara Stephanie Sousa ^[5] ; de Mello, Evandro Sobroza ^[6] ; da Silva Pinhal, Maria Aparecida ^[2] ; Moyses, Raquel Ajub ^[7] ; Kulcsar, Marco Aurelio Vamondes ^[8] ; Dedivitis, Rogerio Aparecido ^[9] ; Cernea, Claudio Roberto ^[9] ; Kowalski, Luiz Paulo ^[9] ; de Matos, Leandro Luongo ^[10]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Head & Neck Surg Dept, Anesthesiol Surg Sci & Perioperat Med, Med Sch, Sao Paulo - Brazil

^[2] Fac Med ABC, Biochem Dept, Santo Andre, SP - Brazil

^[3] Univ Sao Paulo HCFMUSP, Ctr Invest Translac Oncol CTO, Inst Canc Estado Sao Paulo ICESP, Hosp Clin, Fac Med, Sao Paulo - Brazil

^[4] Fac Israelita Ciencias Saude Albert Einstei, Nursery Sch, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Med Sch, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Canc Estado Sao Paulo ICESP, Lab Invest Med 14 LIM14, Pathol Dept, Med Sch, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Head & Neck Surg Dept, Lab Invest Med 28 LIM28, Med Sch, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Head & Neck Surg Dept, Inst Canc Estado Sao Paulo ICESP, Med Sch, Sao Paulo - Brazil

^[9] Univ Sao Paulo, Head & Neck Surg Dept, Med Sch, Sao Paulo - Brazil

^[10] Univ Sao Paulo, Head & Neck Surg Dept, Inst Canc Estado Sao Paulo, Lab Invest Med 28 LIM 28, Med Sch, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK; v. 43, n. 8, p. 2364-2376, AUG 2021.
• Citações Web of Science: 0

Resumo

Background We evaluated microRNAs and extracellular matrix component profiles in squamous cell carcinoma of the oral cavity (OSCC) in comparison to healthy mucosa. Methods Retrospective study investigating 64 microRNAs related to oncogenic process and to constituents of the extracellular matrix. We also performed immunohistochemical assays for molecules involved in the same biological processes. Results High expression of miR-21-5p (p < 0.001) and miR-106-5p (p < 0.001) and low expression of miR-320a (p = 0.001) and miR-222-3p (p = 0.001) were predictors of malignancy. Individually, miR-21-5p exhibited the best statistical performance (area under the curve = 0.972; 95% confidence interval: 0.911-1.000) in the differentiation between tumor tissue and healthy mucosa. Moreover, tumor sample showed increased expression of MMP-2, MMP-9, alpha-laminin, and beta-laminin in tumor-related fibroblasts and lower continuity of type IV collagen in the basement membrane. Conclusion The present study demonstrates the biological effects of microRNAs on the carcinogenesis of OSCC as well as the intense modification of the tumor microenvironment. (AU)

• Processo FAPESP: 16/01740-6 - PERFIL DE PROTEOGLICANOS E EXPRESSÃO DE MICRO-RNAs ASSOCIADOS A INVASÃO TUMORAL E PROGNÓSTICO EM CARCINOMA ESPINOCELULAR DE CAVIDADE ORAL
• Beneficiário: Leandro Luongo de Matos
• Modalidade de apoio: Auxílio à Pesquisa - Regular