| Texto completo | |
| Autor(es): |
Chang, Chia-Chun
[1]
;
Keppeke, Gerson Dierley
[1, 2]
;
Antos, Christopher L.
[1]
;
Peng, Min
[3]
;
Coelho Andrade, Luis Eduardo
[2]
;
Sung, Li-Ying
[3, 4]
;
Liu, Ji-Long
[1, 5]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210 - Peoples R China
[2] Univ Fed Sao Paulo, Rheumatol Div, Escola Paulista Med, BR-04023062 Sao Paulo, SP - Brazil
[3] Natl Taiwan Univ, Inst Biotechnol, Taipei 106 - Taiwan
[4] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115 - Taiwan
[5] Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT - England
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Experimental Cell Research; v. 405, n. 2 AUG 15 2021. |
| Citações Web of Science: | 1 |
| Resumo | |
Cytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-L-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates. (AU) | |
| Processo FAPESP: | 17/20745-1 - Depleção de autoanticorpos por edição gênica com o sistema CRISPR/Cas9 em plasmócitos autorreativos |
| Beneficiário: | Gerson Dierley Keppeke |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |