| Full text | |
| Author(s): |
Chang, Chia-Chun
[1]
;
Keppeke, Gerson Dierley
[1, 2]
;
Antos, Christopher L.
[1]
;
Peng, Min
[3]
;
Coelho Andrade, Luis Eduardo
[2]
;
Sung, Li-Ying
[3, 4]
;
Liu, Ji-Long
[1, 5]
Total Authors: 7
|
| Affiliation: | [1] ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210 - Peoples R China
[2] Univ Fed Sao Paulo, Rheumatol Div, Escola Paulista Med, BR-04023062 Sao Paulo, SP - Brazil
[3] Natl Taiwan Univ, Inst Biotechnol, Taipei 106 - Taiwan
[4] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115 - Taiwan
[5] Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT - England
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Experimental Cell Research; v. 405, n. 2 AUG 15 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Cytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-L-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates. (AU) | |
| FAPESP's process: | 17/20745-1 - Autoantibodies depletion on autoreactive plasmocytes by genome editing with the CRISPR/Cas9 system |
| Grantee: | Gerson Dierley Keppeke |
| Support Opportunities: | Research Grants - Young Investigators Grants |