Starch turnover is stimulated by nitric oxide in embryogenic cultures of Araucaria angustifolia

Autor(es)

Navarro, Bruno V. ^{[1, 2]} ; de Oliveira, Leandro F. ^[1] ; de Oliveira, Lauana P. ^[2] ; Elbl, Paula ^[1] ; Macedo, Amanda F. ^[1] ; Buckeridge, Marcos S. ^[2] ; Floh, Eny I. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Physiol Ecol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT CELL TISSUE AND ORGAN CULTURE; v. 147, n. 3 AUG 2021.
• Citações Web of Science: 1

Resumo

Key message The reduction in starch content caused by nitric oxide scavenger may play an important role in Araucaria angustifolia somatic embryogenesis responsiveness. Embryogenesis is a complex developmental process dependent on the intercommunication of coordinated signals. Among these signals, carbohydrate metabolism seems to be central, modulating responses according to the spatio-temporal environmental variations. Nitric oxide (NO) is a molecule that is extensively involved in various growth processes; however, the regulatory mechanism effect of NO signaling on carbohydrate metabolism is still largely unknown. Thus, in this study, we investigated the interaction between carbohydrate metabolism and NO homeostasis. First, the expression values of sucrose, raffinose, and starch metabolisms target genes were evaluated during Araucaria angustifolia zygotic and early somatic embryo development. This result demonstrated that starch metabolism targets highlighted the differences between zygotic embryo development and progression in early somatic embryo establishment. Therefore, non-structural carbohydrate content, NO emission, and starch metabolism-related genes were evaluated under treatments with donor, scavenger, or inhibitor of NO biosynthesis. These analyses were conducted at the somatic embryogenesis proliferation phase. In this work, the results showed that NO acted on the starch content, and in a different way between cell lines with contrasting responsiveness for the development of early somatic embryos. This indicates that the modification of starch accumulation by NO might be an effective strategy to optimize the in vitro conditions for A. angustifolia somatic embryo development. (AU)

• Processo FAPESP: 14/11228-5 - Integração de redes de sinalização celular e de reguladores de crescimento durante a embriogênese de Araucaria angustifolia
• Beneficiário: Eny Iochevet Segal Floh
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/26888-0 - Marcadores bioquímicos associados ao metabolismo de carboidratos durante a embriogênese zigótica e somática de Araucaria angustifolia (Bert.) O. Ktze.
• Beneficiário: Bruno Viana Navarro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/17541-2 - Do estresse ao embrião: mecanismos moleculares e fisiológicos associados à embriogênese somática
• Beneficiário: Eny Iochevet Segal Floh
• Modalidade de apoio: Auxílio à Pesquisa - Regular