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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Starch turnover is stimulated by nitric oxide in embryogenic cultures of Araucaria angustifolia

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Author(s):
Navarro, Bruno V. [1, 2] ; de Oliveira, Leandro F. [1] ; de Oliveira, Lauana P. [2] ; Elbl, Paula [1] ; Macedo, Amanda F. [1] ; Buckeridge, Marcos S. [2] ; Floh, Eny I. S. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Physiol Ecol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLANT CELL TISSUE AND ORGAN CULTURE; v. 147, n. 3 AUG 2021.
Web of Science Citations: 1
Abstract

Key message The reduction in starch content caused by nitric oxide scavenger may play an important role in Araucaria angustifolia somatic embryogenesis responsiveness. Embryogenesis is a complex developmental process dependent on the intercommunication of coordinated signals. Among these signals, carbohydrate metabolism seems to be central, modulating responses according to the spatio-temporal environmental variations. Nitric oxide (NO) is a molecule that is extensively involved in various growth processes; however, the regulatory mechanism effect of NO signaling on carbohydrate metabolism is still largely unknown. Thus, in this study, we investigated the interaction between carbohydrate metabolism and NO homeostasis. First, the expression values of sucrose, raffinose, and starch metabolisms target genes were evaluated during Araucaria angustifolia zygotic and early somatic embryo development. This result demonstrated that starch metabolism targets highlighted the differences between zygotic embryo development and progression in early somatic embryo establishment. Therefore, non-structural carbohydrate content, NO emission, and starch metabolism-related genes were evaluated under treatments with donor, scavenger, or inhibitor of NO biosynthesis. These analyses were conducted at the somatic embryogenesis proliferation phase. In this work, the results showed that NO acted on the starch content, and in a different way between cell lines with contrasting responsiveness for the development of early somatic embryos. This indicates that the modification of starch accumulation by NO might be an effective strategy to optimize the in vitro conditions for A. angustifolia somatic embryo development. (AU)

FAPESP's process: 14/11228-5 - Integration of cellular signaling and growth regulators networks during embryogenesis in Araucaria angustifolia
Grantee:Eny Iochevet Segal Floh
Support Opportunities: Regular Research Grants
FAPESP's process: 14/26888-0 - Biochemical markers associated with carbohydrate metabolism during Araucaria angustifolia zygotic and somatic embryogenesis
Grantee:Bruno Viana Navarro
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/17541-2 - From stress to embryos: molecular and physiological mechanisms associated to somatic embryogenesis
Grantee:Eny Iochevet Segal Floh
Support Opportunities: Regular Research Grants