Advanced search
Start date
Betweenand

Integration of cellular signaling and growth regulators networks during embryogenesis in Araucaria angustifolia

Grant number: 14/11228-5
Support Opportunities:Regular Research Grants
Start date: November 01, 2014
End date: January 31, 2017
Field of knowledge:Biological Sciences - Botany - Pant Physiology
Principal Investigator:Eny Iochevet Segal Floh
Grantee:Eny Iochevet Segal Floh
Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:André Luiz Wendt dos Santos ; Paula Maria Elbl

Abstract

Embryogenesis is a complex, highly organized biological process that plays a central role in the life cycle of an organism. More specifically for gymnosperms, zygotic and somatic embryogenesis (SE) models have been proven to be promising tools in elementary studies on cell biology, physiology and biochemistry as well as on large-scale vegetative propagation and the establishment of in vitro germplasm banks. Although clonal propagation and ex situ conservation of elite genotypes based on SE is now a consolidated method for a number of gymnosperm species, the adoption of suboptimal conditions during in vitro growth of somatic polyembryos has restricted its use in Araucaria angustifolia. One of the approaches developed to correctly handle in vitro embryos is based on comparative investigations between zygotic and somatic embryogenesis. In line with previous studies conducted in BIOCEL/IB/USP, realized during the last fifteen years, the present research project has as main objective to integrate transcriptome and proteome platforms to study signaling molecules and growth regulators metabolic routes during A. angustifolia embryogenesis. For this purpose physiological, biochemical, and molecular parameters, including the recent established transcriptomic database for embryogenesis in A. angustifolia (FAPESP project proc. 2011/51659-7) will be utilized. Results will be used for metabolic routes study concerning polyamines, auxin, ethylene, nitric oxide, reactive oxygen species, abscísico acid, and salicylic acid. These studies will pave the way for a more in-depth understanding of essential aspects of the plant cell differentiation processes in vitro and in situ, and generate relevant information for the development of efficient somatic embryogenesis protocols for conifers. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NAVARRO, BRUNO V.; DE OLIVEIRA, LEANDRO F.; DE OLIVEIRA, LAUANA P.; ELBL, PAULA; MACEDO, AMANDA F.; BUCKERIDGE, MARCOS S.; FLOH, ENY I. S.. Starch turnover is stimulated by nitric oxide in embryogenic cultures of Araucaria angustifolia. PLANT CELL TISSUE AND ORGAN CULTURE, v. 147, n. 3, . (14/11228-5, 14/26888-0, 16/17541-2)