Interaction between nitric oxide and nitrogen metabolism on root development of model species Arabidopsis thaliana and Brazilian native tree species

• Grant number: 19/15095-3
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2020
• End date: August 31, 2023
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Marília Gaspar Maïs
• Grantee: Marília Gaspar Maïs
• Host Institution: Instituto de Pesquisas Ambientais (IPA). Secretaria de Meio Ambiente, Infraestrutura e Logística (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Amedea Barozzi Seabra ; André Luiz Wendt dos Santos ; Eduardo Purgatto ; Halley Caixeta de Oliveira

Abstract

Nitrate and ammonium are the main inorganic sources of nitrogen for plants and are able to modify root architecture. Different plant species may differ in the nitrogen source preferences and present different responses to nitrate and ammonium. Although nitrate and ammonium can act as signaling molecules, inducing rapidly a large number of genes, root responses to nutrients can be mediated by other molecules, such as nitric oxide (NO). Nitrate reductase (NR) is considered the main enzyme involved in NO synthesis in plants. The enzyme S-nitrosoglutathione reductase (GSNOR), in turn, controls the levels of S-nitrosoglutathione (GSNO), the main NO reservoir in plant cells. This work aims to evaluate if differences in root architecture of Arabidopsis thaliana and native Brazilian tree species grown under nitrate or ammonium would be related to NO / GSNO levels in the plant. For this purpose, seedlings of different native tree species with different nitrogen strategies (Heliocarpus popayanensis, Cariniana estrellensis and Araucaria angustifolia) will be cultivated in hydroponics and the plants of A. thaliana of the wild genotypes (Col-0) and of mutants with changes in NO content (nia1nia2, gsnor + and gsnor-) will be cultured in vitro. In both models, plants will be treated with different nitrogen sources and concentrations. Exogenous application of GSNO and of an NO scavenger in the presence of nitrate or ammonium will also be performed. Morphological parameters (length and mass of main root and lateral roots, root hair density), biochemical parameters (quantification of compounds and activity of enzymes related to nitrate and NO metabolism) and molecular parameters (expression of some genes of interest involved in the observed responses) will be evaluated. Additionally, we expect to identify S-nitrosylated proteins and metabolites whose abundance has been modulated by NO aiming to associate the overall identified modifications with changes in root architecture. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, CAMILLA ALVES; MORO, CAMILA FERNANDES; SALGADO, IONE; BRAGA, MARCIA REGINA; GASPAR, MARILIA. Noncoding RNAs responsive to nitric oxide and their protein-coding gene targets shed light on root hair formation in Arabidopsis thaliana. FRONTIERS IN GENETICS, v. 13, p. 13-pg., 2022-09-27. (11/13220-3, 20/11908-7, 17/50341-0, 19/15095-3)

SEABRA, AMEDEA B.; SILVEIRA, NEIDIQUELE M.; RIBEIRO, RAFAEL, V; PIERETTI, JOANA C.; BARROSO, JUAN B.; CORPAS, FRANCISCO J.; PALMA, JOSE M.; HANCOCK, JOHN T.; PETRIVALSKY, MAREK; GUPTA, KAPUGANTI J.; et al. Nitric oxide-releasing nanomaterials: from basic research to potential biotechnological applications in agriculture. NEW PHYTOLOGIST, v. 234, n. 4, p. 7-pg., 2022-03-29. (20/03646-2, 12/19167-0, 17/11279-7, 18/08194-2, 19/15095-3, 15/21546-7)