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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Germline and Somatic mutations in postmenopausal breast cancer patients

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Autor(es):
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Nagy, Tauana Rodrigues [1] ; Maistro, Simone [1] ; Encinas, Giselly [1] ; Hirata Katayama, Maria Lucia [1] ; de Lima Pereira, Glaucia Fernanda [1] ; Gaburo-Junior, Nelson [2] ; Moyses Franco, Lucas Augusto [3] ; Ribeiro Chaves de Gouvea, Ana Carolina [1] ; Estevez Diz, Maria del Pilar [1] ; Senna Leite, Luiz Antonio [1] ; Azevedo Koike Folgueira, Maria Aparecida [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Dept Radiol & Oncol, Inst Canc Estado Sao Paulo IC, Sao Paulo, SP - Brazil
[2] Diagnost Brasil, DB Mol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med FMUSP, Dept Molestias Infecciosas & Parasitarias, Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Clinics; v. 76, 2021.
Citações Web of Science: 0
Resumo

OBJECTIVES: In breast cancer (BC) patients, the frequency of germline BRCA mutations (gBRCA) may vary according to the ethnic background, age, and family history of cancer. Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) is the second most common somatic mutated gene in BC; however, the association of mutations in both genes with cancer has not been thoroughly investigated. Thus, our aims were to investigate gBRCA mutation frequency in a cohort of postmenopausal Brazilian BC patients and the association of gBRCA1/BRCA2 and PIK3CA somatic mutations. METHODS: Forty-nine postmenopausal (>55 years) and forty-one young (<= 35 years) BC patients were included in this study. The postmenopausal group included patients who reported a positive family history of cancer. For these patients, gBRCA1/BRCA2 were sequenced using next-generation sequencing (NGS) or Sanger sequencing. Data for gBRCA in young patients were already available from a previous study. DNA from formalin-fixed, paraffin-embedded (FFPE) tumors was obtained from 27 postmenopausal and 41 young patients for analyzing exons 9 and 20 of PIK3CA. The association between gBRCA1/BRCA2 and somatic mutations in PIK3CA was investigated. RESULTS: The overall frequency of gBRCA1/BRCA2 among the 49 postmenopausal patients was 10.2%. The frequencies of somatic mutations in PIK3CA in the postmenopausal and young patients were 37% and 17%, respectively (ns). The most common PIK3CA mutation was found to be E454A. Nonsense and frameshift mutations, which may counteract the oncogenic potential of PIK3CA were also detected. Regardless of age, 25% of BRCA1/BRCA2 mutation carriers and non-carriers , each, had PIK3CA somatic mutations. CONCLUSIONS: Data obtained indicate that BRCA1/BRCA2 gene testing may be considered for postmenopausal patients with BC who have a family history of cancer. Although some of them are not considered pathogenic, somatic variants of PIK3CA are frequently observed in BC patients, especially in postmenopausal patients. (AU)

Processo FAPESP: 11/09572-1 - Determinação de Mutações Somáticas e Germinativas em Pacientes Jovens com Câncer de Mama
Beneficiário:Giselly Encinas Zanetti
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/12306-4 - Determinação de mutações somáticas e germinativas em pacientes jovens com câncer de mama
Beneficiário:Maria Aparecida Azevedo Koike Folgueira
Linha de fomento: Auxílio à Pesquisa - Regular