Tyrosine and Tryptophan vibrational bands as markers of kidney injury: a renocardiac syndrome induced by renal ischemia and reperfusion study

Autor(es)

Nepomuceno, Gabrielle ^[1] ; Cruz Junho, Carolina Victoria ^[1] ; Carneiro-Ramos, Marcela Sorelli ^[1] ; Martinho, Herculano da Silva ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 JUL 22 2021.
• Citações Web of Science: 0

Resumo

Renal injury caused by renal ischemia and reperfusion strongly influences heart morphology, electrophysiology, and redox unbalance. The so-called cardiorenal syndrome is an important class of dysfunction since heart and kidneys are responsible for hemodynamic stability and organ perfusion through a complex network. In the present work we investigate the vibrational spectral features probed by Fourier-Transform Raman (FT-Raman) spectroscopy due to physiological alterations induced by renal ischemic reperfusion aiming to detect molecular markers related to progression of acute to chronic kidney injury and mortality predictors as well. C57BL/6J mice were subjected to unilateral occlusion of the renal pedicle for 60 min and reperfusion for 5, 8, and 15 days. Biopsies of heart and kidney tissues were analyzed. Our findings indicated that cysteine/cystine, fatty acids, methyl groups of Collagen, alpha -form of proteins, Tyrosine, and Tryptophan were modulated during renal ischemia and reperfusion process. These changes are consistent with fibroblast growth factors and Collagen III contents changes. Interestingly, Tyrosine and Tryptophan, precursor molecules for the formation of uremic toxins such as indoxyl sulfate and p-cresyl sulfate were also modulated. They are markers of kidney injury and their increase is strongly correlated to cardiovascular mortality. Regarding this aspect, we notice that monitoring the Tyrosine and Tryptophan bands at 1558, 1616, and 1625 cm(-1) is a viable and and advantageous way to predict fatality in cardiovascular diseases both ``in vivo{''} or ``in vitro{''}, using the real-time, multiplexing, and minimally invasive advantages of FT-Raman spectroscopy. (AU)

• Processo FAPESP: 19/11077-0 - Alterações cardíacas induzidas por modelos de inflamação renal: participação do eixo Klotho/FGF-23
• Beneficiário: Marcela Sorelli Carneiro Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/19924-2 - Estudo e desenvolvimento de novos materiais avançados: eletrônicos, magnéticos e nanoestruturados: uma abordagem interdisciplinar
• Beneficiário: Carlos Rettori
• Modalidade de apoio: Auxílio à Pesquisa - Temático