volution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animal

Autor(es)

Bezerra, Bruno Santos ^[1] ; Belato, Flavia Ariany ^[1] ; Mello, Beatriz ^[2] ; Brown, Federico ^[1] ; Coates, Christopher J. ^[3] ; Leme, Juliana de Moraes ^[4] ; Trindade, Ricardo I. F. ^[5] ; Costa-Paiva, Elisa Maria ^{[5, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Zool, Sao Paulo - Brazil

^[2] Univ Fed Rio de Janeiro, Biol Inst, Genet Dept, Rio De Janeiro - Brazil

^[3] Swansea Univ, Fac Sci & Engn, Dept Biosci, Swansea, W Glam - Wales

^[4] Univ Sao Paulo, Geosci Inst, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Astron Geophys & Atmospher Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 AUG 3 2021.
• Citações Web of Science: 1

Resumo

The biological toolkits for aerobic respiration were critical for the rise and diversification of early animals. Aerobic life forms generate ATP through the oxidation of organic molecules in a process known as Krebs' Cycle, where the enzyme isocitrate dehydrogenase (IDH) regulates the cycle's turnover rate. Evolutionary reconstructions and molecular dating of proteins related to oxidative metabolism, such as IDH, can therefore provide an estimate of when the diversification of major taxa occurred, and their coevolution with the oxidative state of oceans and atmosphere. To establish the evolutionary history and divergence time of NAD-dependent IDH, we examined transcriptomic data from 195 eukaryotes (mostly animals). We demonstrate that two duplication events occurred in the evolutionary history of NAD-IDH, one in the ancestor of eukaryotes approximately at 1967 Ma, and another at 1629 Ma, both in the Paleoproterozoic Era. Moreover, NAD-IDH regulatory subunits beta and gamma are exclusive to metazoans, arising in the Mesoproterozoic. Our results therefore support the concept of an `'earlier-than-Tonian'' diversification of eukaryotes and the pre-Cryogenian emergence of a metazoan IDH enzyme. (AU)

• Processo FAPESP: 16/06114-6 - O Sistema Terra e a evolução da vida durante o Neoproterozoico
• Beneficiário: Ricardo Ivan Ferreira da Trindade
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/18051-7 - Respostas fisiológicas, morfológicas e do desenvolvimento de metazoários modernos submetidos às condições oceânicas do Neoproterozóico
• Beneficiário: Flávia Ariany Belato Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/20268-1 - Fisiologia de Análogos Modernos da fauna Ediacarana e Cambriana
• Beneficiário: Elisa Maria Costa e Silva de Paiva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado