Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam

Autor(es)

Decio, Pamela ^[1] ; Miotelo, Lucas ^[1] ; Pereira, Franco Dani Campos ^{[1, 2]} ; Roat, Thaisa Cristina ^[1] ; Marin-Morales, Maria Aparecida ^[1] ; Malaspina, Osmar ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Inst Biosci, Ave 24A, 1515, BR-13506900 Rio Claro, SP - Brazil

^[2] NUPEFEN Nucleo Pesquisas Educ Fis Estet & Nutr, Claretiano Univ Ctr, Ave Santo Antonio Maria Claret, BR-13503257 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 223, OCT 15 2021.
• Citações Web of Science: 0

Resumo

The increasing use of insecticides, promoted by the intensification of agriculture, has raised concerns about their influence on the decline of bee colonies, which play a fundamental role in pollination. Thus, it is fundamental to elucidate the effects of insecticides on bees. This study investigated the damage caused by a sublethal concentration of thiamethoxam - TMX (0.0227 ng/mu L of feed) in the head and midgut of Africanized Apis mellifera, by analyzing the enzymatic biomarkers, oxidative stress, and occurrence of lipid peroxidation. The data showed that the insecticide increased acetylcholinesterase activity (AChE) and glutathione-S-transferase (GST), whereas carboxylesterase (CaE3) activity decreased in the heads. Our results indicate that the antioxidant enzymes were less active in the head because only glutathione peroxidase (GPX) showed alterations. In the midgut, there were no alkaline phosphatase (ALP) or superoxide dismutase (SOD) responses and a decrease in the activity of CaE was observed. Otherwise, there was an increase in GPX, and the TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The results showed enzymes such as CaE3, GST, AChE, ALP, SOD, and GPX, as well as the TBARS assay, are useful biomarkers on bees. They may be used in combination as a promising tool for characterizing bee exposure to insecticides. (AU)

• Processo FAPESP: 14/23197-7 - Estresse celular e atividade de enzimas biomarcadoras em abelhas africanizadas Apis mellifera Lineu, 1758 (Hymenoptera, Apidae) expostas ao tiametoxam
• Beneficiário: Pâmela Decio Horst
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/21833-0 - Proteção de polinizadores: toxicidade do tiametoxam para Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini)
• Beneficiário: Lucas Miotelo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/21097-3 - Interações abelha-agricultura: perspectivas para a utilização sustentável
• Beneficiário: Osmar Malaspina
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 12/13370-8 - Como o cérebro de Apis mellifera (Lineu, 1758) (Hymenoptera, Apidae) responde à dose subletal de tiametoxam?
• Beneficiário: Thaisa Cristina Roat
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores